Cellular Identification and Quantification of Senescence-Associated β-Galactosidase Activity In Vivo: Methods and ProtocolsCellular senescence, Transmission electron microscopy, Senescence-associated β-galactosidaseSenescence-associated β-galactosidase (hereafter SA-β-gal) staining has now been employed for ...
Interlinked and fundamental aging processes appear to be a root-cause contributor to many disorders and diseases. One such process is cellular senescence, which entails a state of cell cycle arrest in response to damaging stimuli. Senescent cells can ari
Three dermal fibroblast cell lines were induced to senescence by IR or passaged until they reached replicative senescence (RS) (Extended Data Fig.1a–c). To confirm that the IR-treated cells were senescent, we evaluated levels of senescence markers p16Ink4a, p21Cip1, p53 and interleukin-6 (...
Cellular senescence was assessed utilizing the senescence-associated β-galactosidase (SA-β-Gal) assay. Furthermore, the effect of METTL3 on SIRT1 mRNA modification was demonstrated, and its mechanism was elucidated using RT-qPCR, western blot, RNA stability assays, and RIP analysis. Results ...
Melatonin attenuates dental pulp stem cells senescence due to vitro expansion via inhibiting MMP3. Oral Dis. 2024;30(4):2410–24. Article CAS PubMed Google Scholar Sui BD, Zheng CX, Zhao WM, Xuan K, Li B, Jin Y. Mesenchymal condensation in tooth development and regeneration: a focus ...
Increased NR-HSCs in aged mice can be explained by an increase in apoptosis, senescence or homing defects due to the accumulation of DNA damage as well as mitochondria and/or other key cellular organelles becoming defective. However, the precise origin of My-bi and Plt-bi HSCs in old mice ...
Age-related accumulation of lipofuscin in the human body, in particular in the brain, correlates with senescence and sturdiness of human tissues (Moreno-García et al., 2018). Similarly, insolubility of the collagen also increases in the human body with aging, leading to hardening, browning, ...
Many of stem cell characteristics in BMSCs were lost after expansion in vitro due to spontaneous differentiation or senescence [4] (Table 1). Table 1. Abbreviations and full names. Empty CellAbbreviationFull name Decellularization methods T Triton X-100 FFF Three freeze/thaw cycles TFFF Triton ...
In the first phase, we combine the measurement of lysosomal and proliferative features with the expression of general senescence-associated genes to validate the presence of senescent cells. In the second phase we measure the levels of pro-inflammatory markers for specification of the type of ...
Senescent vascular cells are detected in atherosclerotic lesion, and its involvement in the development of atherosclerosis has been revealed; however, whether and the mechanism by which endothelial cell (EC) senescence is causally implicated in atherosclerosis remains unclear. We here investigate a role ...