Cell‐free fetal DNA (cffDNA)DIP‐STRNoninvasive prenatal paternity testing (NIPAT)Selective amplification technologySNP‐STRWith the first discovery of circulating cell‐free fetal DNA (cffDNA) in maternal plasma, non﹊nvasive prenatal testing became possible. However, analysis of low﹍evel cffDNA...
1) cell-free fetal DNA 胎儿游离DNA 1. Detection ofcell-free fetal DNAin maternal serum for a new prenatal screening marker of Down s syndrome; 孕妇血清中胎儿游离DNA产前筛查Down’s综合征初探 2. Influence of delay in blood-processing on the level ofcell-free fetal DNAin maternal plasma sampl...
(3) 40 cases of preeclampsia were positive,concentration of mild preeclampsia was 2.39 times of the normal third-trimester,and concentration of severe preeclampsia was 5.78 times of the normal third-trimester.Conclusion Contect of cell-free fetal DNA in maternal plasma increase as pregnancy advances,...
The value of the detection of cell-free fetal DNA in maternal plasma in the preeclampsia women using fluorescent quantitative PCR 荧光定量PCR检测孕妇外周血中游离胎儿DNA在子痫前期中的价值 www.ilib.cn4. Cell-free fetal DNA in maternal plasma and its clinical significance 孕妇血浆中的游离胎儿DNA及其...
ObjectiveTo determine the sensitivity and specificity of circulating cell-free fetal DNA in determining the fetal RHD status and fetal sex. MethodsMaternal blood was collected in each trimester of pregnancy from RhD negative nonalloimmunized women. Whole blood was centrifuged, separated into plasma an...
The fetal fraction, or percentage of total cell-free DNA (cfDNA) in a sample derived from the fetus, can impact NIPT results and profitability.
using fetal epigenetic changes such as DNA methylation and messenger RNA (mRNA) expressed from the placenta. However, these approaches had significant technical disadvantages, such as the destructive nature of many methylation detection methods and the lability of mRNA, and consequently were not practi...
DNA samples,of which 17 cases were verified by paternal DNA.Conclusion:Touch-down PCR and boster PCR assays could improve the amplification efficiency of low-level fetal DNA during early pregnancy,and the fetal genetic information which independent on paternal DNA could be obtained accurately and ...
NoneJohn Wiley & Sons, Ltd.American Journal of Medical Genetics Part ACell-free fetal DNA tests for trisomy show promise in women at lower risk of affected pregnancies. American Journal of Medical Genetics Part A 164 :10.1002/ajmg.a.v164a.6, viii-ix /...
aCell-free fetal nucleic acids (cffNA) can be detected in the maternal circulation during pregnancy, potentially offering an excellent method for early non-invasive prenatal diagnosis (NIPD) of the genetic status of a fetus. Using molecular techniques, fetal DNA and RNA can be detected from 5 ...