The simulated ST data, derived from the scRNA dataset with malignant cells, has three different malignant cell fractions, i.e., 0% (500 spots), 50% (200 spots), and 100% (500 spots). For a spot, 1–3 cell types and 3–10 cells were randomly selected from the scRNA-seq dataset ...
Be arranged to differentiate into other units (such as epidermal cell and nerve cell) from the multipotential cell straight line II of cancerous tissue except the unit in cancer stem cell except in cancerous tissue, and individually, so as to it is highly useful be the treatment of various...
between cell lines derived from different cell types or cell lines that may not be representative of PAAD. Of potential concern, the cell line with the lowest median correlation coefficient, MIA PaCa-2, is commonly used as an adenocarcinoma cell line model and has over 1000 PubMed citations....
The 1-LN cell line (a kind gift from Dr. Phillip Walther, Duke University Medical Center) is a highly metastatic cell line derived from the less metastatic PC-3 cell line. The 1-LN cell line expresses high levels of GRP78 on the cell-surface, while PC-3 cells do not express cell-su...
To further explore the heterogeneity of B-cell subtypes, we performed a cluster analysis of B cells derived from different tissues for each cluster (Fig. 3A). In addition, we counted the number and percentage of each B-cell subtype from different tissues. Although all 12 B-cell subtypes were...
Human embryonic stem cells (hESCs) are derived from the ICM. During the process of embryogenesis, cells form aggregations called germ layers: endoderm, mesoderm and ectoderm (Fig. 1), each eventually giving rise to differentiated cells and tissues of the foetus and, later on, the adult ...
1). To prevent normal cells from outgrowing cancer cells, culture conditions need to be selective or organoids initiated exclusively with tumour cells36. In contrast to organoids derived from normal bronchial epithelial cells, NSCLC-derived 3D cultures produce more solid spheres lacking apico-basal ...
The establishment and characterisation of paired autologous tumour cell line (MST-1) and tumour-infiltrating lymphocyte (TIL) culture from a tumour mass of a 14-year-old Taiwanese girl with soft tissue melanoma are described. MST-1 cells grown in vitro were heterogeneous in morphology, ranging fr...
T cells (derived from female mice) were adoptively transferred approximately seven days after tumour inoculation, or when tumours became palpable. Specifically, 5 × 106 sorted T cells were resuspended in 50 μl of PBS per mouse and administered through retroorbital injection. Where ...
The STC-1 cell line that was derived from an endocrine tumour of the murine intestine was kindly provided by Prof. Douglas Hanahan from the University of California. The STC-1 cells were cultured in DMEM (Dulbecco's Modified Eagle's Medium; PAA, Austria) containing 10% FBS (Fetal bovine ...