To optimize patient prognosis, patient-derived tumor-like cell clusters (PTC) for drug testing seeks to identify an effective drug for individual patient.Methods:A prospective, single-center, parallel-group, randomized phase II clinical trial was conducted from December 2020 to December 2023 in ...
Over the past decade, the emergence of patient-derived tumor organoids (PDTOs) has broadened the repertoire of preclinical models and progressively revolutionized three-dimensional cell culture in oncology. PDTO can be grown from patient tumor samples with high efficiency and faithfully recapitulates th...
Patient-derived bladder cancer xenografts: a systematic review. Transl Res. 166, 324–331 (2015). Article PubMed Google Scholar Skowron, K. B. et al. Basal tumor cell isolation and patient-derived xenograft engraftment identify high-risk clinical bladder cancers. Sci. Rep. 6, 35854 (2016)...
Cell type deconvolution: CIBERSORTx [20] was used to infer fractions and expression profiles of different cell types in each sample. A signature matrix was built from scRNA-seq data of human breast cancers [21] analyzed with Seurat v5 [22]. After clustering, cell clusters not assigned to B ...
Patient-derived tumor cell organoid culture mimic parental tumors From a histopathological perspective, PDX models largely retain parent tumor architecture, as described above. Further, at the cellular level, both inter-tumoral and intra-tumoral heterogeneity, as well as the phenotypic and molecular char...
Effect of patient-derived tumor-like cell clusters on personalized medicine of patients with non-muscle invasive bladder cancer. e16610#Background:Non-muscle invasive bladder cancer (NMIBC) has a high recurrence and progression rate, and the intravesical instillation chemotherapy dru... J Xi,S Yin...
To achieve this critical aim, reliable patient-derived brain tumor models are needed. The lack of in-depth comprehension of preclinical models is regularly stated as one of the main challenges in curing cancer, including brain tumors [15]. Despite being used for functional investigations of the ...
(F) Tumor formations by primary liver cancer cells and LCSCs (Left panel). Vascular marker CD31(red) was detected by immunofluorescence staining in LCSC-derived xenograft and primary liver cancer cells (Right). Scale bar 50 μm. (G) The bar graph displayed the statistical immunofluorescent ...
and samples, based on log-transformed CPM expression values in 11 PDCs, with the SciPy Python library [30]. Expression values were normalised on a zero-to-one scale, and the derived clusters were then visualised using the Seaborn Python library as described above. Fusion genes were predicted ...
aFor one patient, organoid lines were derived from the primary tumor (P) as well as two liver metastases (M1 and M2). Shown is a UMAP representation of transcriptomes integrated by reciprocal PCA, with shared and organoid line-specific cell clusters identified by unsupervised clustering. Cells ...