近日，挪威约翰英纳斯中心Saskia A. Hogenhout课题组在Cell上发表题为Parasitic modulation of host development by ubiquitin-independent protein degradation的学术论文，本文发现了植原体效应因子SAP05可以靶标和降解两个不同的转录因子家族，SPL家族和GATA家族，从而导致植物生长周期增长和丛枝病等症状。SAP05通过与26S泛...
Analysis of components required for protein folding, protein stability or protein degradation The diagram provides an example of application of a cell-free protein expression method combined with protein mass spectrometry (MS). Workflow ...
Cell-free protein synthesis is a versatile protein production system. Performance of the protein synthesis depends on highly active cytoplasmic extracts. Extracts fromE. coliare believed to work best; they are routinely obtained from exponential growing cells, aiming to capture the most active translatio...
2.3. Wheat germ cell-free protein synthesis of CrdS Wheat germ cell-free protein synthesis (WG-CFPS) of CrdS was performed sequentially through the uncoupled transcription and translation reactions. Care was taken to prevent RNAse contamination throughout by using RNase-free water (MP Biomedicals,...
Protein degradation through ubiquitin-mediated proteolysis plays an important role in the cell-cycle regulation. Most importantly, the Anaphase Promoting Complex/Cyclosome (APC/C) and the Skp1-Cullin-1-F-box complex (SCF) are the two major E3 ubiquitin ligase complexes that regulate proper cell ...
Cell-free protein synthesis has been widely used as a “breadboard” for design of synthetic genetic networks. However, due to a severe lack of modularity, forward engineering of genetic networks remains challenging. Here, we demonstrate how a combination of optimal experimental design and microfluidi...
According to the proposed model, degradation of circRNAs by activated RNase L is perhaps a prerequired step to free PKR from the bound circRNAs. Although to address each of these questions will be a challenging, prospective topic, the reported study definitely opens a new avenue guiding the next...
Protein synthesis by35S-cysteine–methionine incorporation Protein synthesis in cell-free purified fractions ofE.chaffeensiswas measured by incorporation of35S-Cys-Met (Perkin Elmer, Waltham, MA) as described previously20. For normalization of bacterial total protein content, the suspensions ofE.chaffeensi...
2024年3月12日,华东师范大学叶海峰团队在《Molecular Cell》发表题“A programmable targeted protein-degradation platform for versatile applications in mammalian cells and mice”的研究论文。该项研究进一步实现了靶向蛋白质降解领域的可调控和可编程应用。
针对这些问题,2024年3月12日,华东师范大学生命科学学院、上海市调控生物学重点实验室、医学合成生物学研究中心的叶海峰研究团队在《Molecular Cell》杂志上发表了题为“A programmable targeted protein degradation platform for versatile applica...