02无创检测 Amplification-free sequencing of cell-free DNA for prenatal non-invasive diagnosis of chromosomal aberrations.Karlsson K,et al.Genomics.2014 Dec 24. 03其他疾病及症状 High genetic diversity of equine infectious anaemia virus strains from Slovenia revealed upon phylogenetic analysis of the p15...
Protein concentration was determined using the Qubit Protein Assay Kit (Invitrogen) or by the Protein Assay Kit, based on a Coomassie Brilliant Blue G-250 dye shift (Bio-Rad) [2]. Precision Plus protein molecular mass standards were from Bio-Rad. Purity of CrdS preparations resolved by IMAC...
We also confirmed the specific roles of GA receptors and multisubunit E3 ligase components in regulating DELLA protein degradation. In addition, blocking DELLA degradation with a PP1/PP2A phosphatase inhibitor in our cell-free assay suggested that degradation of DELLA proteins required protein Ser/Thr...
free whole-genome methylation sequencing method cfTAPS30. Unlike cfTAPS which employed a lengthy incubation step with pyridine borane to convert 5caC to DHU, the enzyme-based WMS assay is anticipated to be more robust because it is potentially less sensitive to fluctuations in experimental ...
and presence of thiol was confirmed by Ellman’s assay. A cyclooctyne handle was then introduced using DBCO-Maleimide to react with the free thiol. DBCO-Maleimide was dissolved at 10 mg/mL in DMSO, and 10 eq of this stock solution was added to 1 mL of CpG-SH in PBS. The reaction mix...
protein expression of H3K9me3 in human CD8+T cells treated with or without 500 µM DEG or 500 µM αKG for 7 days. Ordinary one-way ANOVA;n = 3–5. DEG,n = 11; αKG,n = 3; αKG + DEG,n = 3.i, Cell-free enzymatic inhibition assay for KDM...
Multinucleated osteoclasts, essential for skeletal remodeling in health and disease, are formed by the fusion of osteoclast precursors, where each fusion event raises their bone-resorbing activity. Here we show that the nuclear RNA chaperone, La protein
In vitro chondrogenic differentiation assay For chondrogenic differentiation in high-density pellet cultures, 2.5 × 105of BMSCs were centrifuged to form a pellet. PMI and PTK hydrogels (10 mg/mL) were incubated with defined serum-free chondrogenic medium for 12 h, which was used to co...
). More importantly, no low molecular weight band corresponding to free HiBiT peptide was observed, indicating that the luminescent signal measured in the SLEEQ assay comes from intact fusion protein, and not degradation products of the protein....
针对这些问题,2024年3月12日,华东师范大学生命科学学院、上海市调控生物学重点实验室、医学合成生物学研究中心的叶海峰研究团队在《Molecular Cell》杂志上发表了题为“A programmable targeted protein degradation platform for versatile applica...