N.benthamiana叶片和香蕉皮的瞬时转化分析 Immunoprecipitation and mass spectrometry (IP-MS) assay\Co-immunoprecipitation and ubiquitination assays\Yeast two-hybrid, pull-down, co-localization and split-luciferase assays In vitro ubiquitination assay\Cell-free degradation assay\In vivo protein degradation assay...
Ubiquitin-Proteasome Protocols Volume 301 || Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation C Patterson,DM Cyr 被引量: 0发表: 2005年 Assaying Proteasomal Degradation in a Cell-free System in Plants The ubiquitin-proteasome pathway for protein degradation has emerged as one ...
In addition, the Click-iT EdU cell proliferation assay can be multiplexed with surface and intracellular marker detection using Alexa Fluor dye–labeled secondary antibodies (Secondary Immunoreagents—Section 7.2) (Figure 15.4.2). A...
A cell-free reaction in which endogenous STING is phosphorylated by recombinant TBK1 To directly assess the roles of Golgi lipids in STING activation, we sought to develop a cell-free assay in which endogenous STING is phosphorylated by TBK1. To eliminate any contribution of cytosolic/endogenous TB...
Figure 1 shows five representative samples of proteins that were precipitated equivalently irrespective of RNase treatment, as well as five representative proteins that were precipitated less effectively subsequent to RNase degradation of the lysate. Both lists of proteins were quantitatively compared and ...
The naked mole rat (NMR), Heterocephalus glaber, the longest-living rodent, provides a unique opportunity to explore how evolution has shaped adult stem cell (ASC) activity and tissue function with increasing lifespan. Using cumulative BrdU labelling and
(CCK-8) to detect the effect of FTL on cell vitality. Compared to the control group, the cell vitality of FTL-knockdown group was significantly decreased (Fig.9d). We next performed the cellular senescence assay, SA-β-gal staining results showed that BEAS-2B cells were induced to ...
Cell-free supernatant and > 50 kDa supernatant fraction present cytostatic activity Our preliminary screen was performed using the MTS assay to evaluate the influence on cell metabolism of cell-free supernatants (CFSs) derived from three Lactococcus lactis ssp. lactis strains, grown under conditions...
There are several areas where Nanopore methylation sequencing may provide unique strengths over other methods. Bisulfite-based sequencing leads to DNA fragmentation and degradation and can obscure fragmentation patterns [41]. Additionally, bisulfite sequencing can not distinguish between 5mC and other modif...
Previous studies have indicated that long-term exposure to an inflammatory microenvironment plays a critical role in the degradation of the extracellular matrix, inhibition of matrix synthesis, and occurrence and development of osteoarthritis [31]. Macrophages, which are important immune regulatory cells,...