N.benthamiana叶片和香蕉皮的瞬时转化分析 Immunoprecipitation and mass spectrometry (IP-MS) assay\Co-immunoprecipitation and ubiquitination assays\Yeast two-hybrid, pull-down, co-localization and split-luciferase assays In vitro ubiquitination assay\Cell-free degradation assay\In vivo protein degradation assay...
Influence of freshwater discharge on the microbial degradation processes of dissolved organic nitrogen in a subtropical estuary.Garcia JC,et al.Antonie Van Leeuwenhoek.2014 Dec 27. --- The genomic landscape of phaeochromocytoma.Flynn A,et al.J Pathol.2014 Dec 26. --- PCR Primers to Study th...
free whole-genome methylation sequencing method cfTAPS30. Unlike cfTAPS which employed a lengthy incubation step with pyridine borane to convert 5caC to DHU, the enzyme-based WMS assay is anticipated to be more robust because it is potentially less sensitive to fluctuations in experimental ...
The investigation of cfDNA has several benefits that have contributed to its growing utility and popularity in medical practice2,5,12,13. Assays of cfDNA are nonetheless sensitive to genomic DNA (gDNA) contamination derived from lysed cells in poorly manipulated samples, cfDNA degradation and the pr...
Nevertheless, whole cell approaches also bear disadvantages like intracellular degradation reactions, transport limitations as well as low substrate solubility. In this work the hydantoinase and carbamoylase from Arthrobacter crystallopoietes DSM 20117 were investigated with respect to their applicability in...
cdt1 and geminin. ubiquitin e3 ligases add ubiquitin to the cdt1–gfp and geminin–rfp fusions, thereby targeting these proteins for proteasomal degradation. temporal regulation of e3 ligase activity results in the biphasic cycli...
Cells were cultured for the indicated time before adding 100 μL of the CellTiter-Glo® luminescence assay reagent in each well. Cells were incubated for an additional 10 min at room temperature to stabilize luminescent signals and transferred to 96-well black plates. Measurements were performed ...
CPProtectides: Rapid uptake of well-folded β-hairpin peptides with enhanced resistance to intracellular degradation Safa N, Anderson J, Vaithiyanathan M, Pettigrew J, Pappas G, et. al. Peptide Science 2019 vol: 111 (2) pp: e24092 Ectopic expression of olfactory receptors and as...
Cell proliferation and viability assay Cells were seeded into 96-well plate (5000 cells/well) with 100 μL complete medium. We added 10 μL Cell Counting Kit-8 (CCK-8; Dojindo, Japan) to the wells at 24 h, 48 h, 72 h and 96 h, respectively, to detect the proliferation of cells....
coli [8], alleviates toxic effects of recombinant protein production on host cells, prevents target proteins from proteolytic degradation and accelerates expression of proteins in a soluble form [9]. The inclusion bodies produced in E. coli were refolded using a range of buffers and non-...