Degradation tags, otherwise known as degrons, are portable sequences that can be used to alter protein stability. Here, we report that degron-tagged proteins compete for cellular degradation resources in engineered mammalian cells leading to coupling of
The recognition of such (normally shielded) hydrophobic residues is the suggested mechanism by which proteasome catalyzes the selective removal of oxidatively modified cell proteins. By minimizing protein aggregation and cross-linking and by removing potentially toxic protein fragments, proteasome plays a ...
Here, we present a novel two-component, blue light-responsive optogenetic OFF switch ('Blue-OFF'), which enables a rapid and quantitative down-regulation of a protein upon illumination. Blue-OFF combines the first light responsive repressor KRAB-EL222 with the protein degradation module B-LID (...
The recognition of such (normally shielded) hydrophobic residues is the suggested mechanism by which proteasome catalyzes the selective removal of oxidatively modified cell proteins. By minimizing protein aggregation and cross-linking and by removing potentially toxic protein fragments, proteasome plays a ...
Resuspend in 50 µl Cell Lysis Buffer. Other cell lysis buffers are suitable. Vortex. Incubate cell suspension at 37°C for 10 minutes to lyse the cells. Note: You may prefer to lyse the cells at room temperature or on ice ...
Other cell lysis buffers are suitable. Vortex. Incubate cell suspension at 37°C for 10 minutes to lyse the cells. Note: You may prefer to lyse the cells at room temperature or on ice if degradation of your protein is a potent...
Thirteen cells were fixed in the GV stage, 25 cells were fixed within 30 min of NEBD and 1 cell was fixed 1 h after NEBD. The remaining 17 oocytes had NEBD shortly before they were obtained from the IVF clinic. All 56 human oocytes were co-stained with an anti-α-tubulin ...
Cell Biol. 45, 1302–1313 (2013). Article CAS PubMed Google Scholar Ying, M. et al. The E3 ubiquitin protein ligase MDM2 dictates all-trans retinoic acid-induced osteoblastic differentiation of osteosarcoma cells by modulating the degradation of RARα. Oncogene 35, 4358–4367 (2016). ...
Fig. 4: The AID2 system can generate conditional mutant cell lines for genes that were not possible to modify with the original AID system. Full size image Our group and Ikonen’s group previously reported that AtAFB2, a TIR1 paralog inArabidopsis thaliana, shows less basal degradation and ...
The AID system can control the abundance of a diverse set of cellular proteins, including those contained within protein complexes, and is active in all phases of the cell cycle. Here we discuss considerations for the successful implementation of the AID system and describe a protocol using ...