Autophagic degradation of ubiquitinated protein aggregates is important for cell survival, but it is not known how the autophagic machinery recognizes such aggregates. In this study, we report that polymerization of the polyubiquitin-binding protein p62/SQSTM1 yields protein bodies that either reside ...
The degradation studies show that MEN2B has a lower level of protein expression and protein degradation, compared to wild type RET. The decrease in RET protein levels inversely correlates with levels of the ubiquitin pathway enzyme, Cbl. The connection between decreased MEN2B down regulation and ...
The amount of protein on the blot was quantified by using ImageQuant software version 5.2 (Molecular Dynamics, Sunnyvale, CA). The percentage of PK-resistant PrP aggregates in each sample was calculated by dividing the amount of protease-resistant rPrPSc in the NaPTA pellet by the total amount...
Two orthorhombic forms (Vm values are 2.3 and 3.0 A3/Da) of bovine beta-trypsin crystals in neat cyclohexane were determined to 1.93 A resolution, by X-ray diffraction. Both structures in organic solvent are similar to those in aqueous solution. In the high packing density form, one cyclohexa...
2 Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway Autophagic degradation of ubiquitinated protein aggregates is important for cell survival, but it is not known how the autophagic machinery recognizes such aggregates. In this study, we report that polymerizat...
Protein transport into the endomembrane system is a particular example. Within the secretory pathway, proteins are transported from the ER involved in the control of proper folding and N-glycosylation, to the vacuole or the extracellular compartment. This transport occurs via the Golgi apparatus that...
Influence of calcium on the proteolytic degradation of the calmodulin-like skin protein (calmodulin-like protein 5) in psoriatic epidermis The calmodulin-like skin protein (CLSP) or so-called calmodulin-like protein 5, a recently discovered skin-specific calcium-binding protein, is closely rel... ...
We report our findings on the assembly of the HIV-1 protein Vpu into soluble oligomers. Vpu is a key HIV-1 protein. It has been considered exclusively a single-pass membrane protein. Previous observations show that this protein forms stable oligomers in
of Mitogen-activated Protein Kinase Kinase Selectively Inhibits Cell Proliferation in Human Breast Cancer Cells Displaying Enhanced Insulin-like Growth Factor I-mediated Mitogen-activated Protein 热度: the presence of an insulin-like androgenic gland factor (iag) and insulin-like peptide binding protein ...
The presence of alpha-synuclein, Parkin and UCH-L1 in Lewy bodies suggests that dysfunction of pathways involved in protein folding and degradation is not only involved in the pathogenesis of familial Parkinson's disease, but could also play a role in the frequent sporadic form of the disease ...