The first step in protein analysis is cellular extraction, which requires liberation of protein from the sample source. Whether using mechanical or detergent-based extraction methods, this process inevitably disrupts cellular homeostasis and contributes to the degradation or ...
Occurrence of the protein degradation in eukaryotic cells; What are lysosomes involve in; Demonstration of the autophagic degradation of organelles.SakaiYasuyoshiKollerAntoniusRangellLindaK.KellerGilbertA.Journal of Cell BiologySakai Y, Koller A, Rangell LK, Keller GA, Subramani S. Peroxisome degradation...
The kinetics of the initial phases of d-glucose binding to the glucose transport protein (GLUT1) of the human red cell can be followed by stopped-flow measurements of the time course of tryptophan (trp) fluorescence enhancement. A number of control experiments have shown that the trp fluorescen...
Degradation begins inside macrophages of the spleen, which remove old and damaged (senescent) erythrocytes from circulation. RBCs are engulfed by cells of the reticuloendothelial system. The globin is recycled into amino acids, which are catabolized into intermediates of the citric acid cycle and fa...
- 《Journal of Clinical Investigation》 被引量: 0发表: 2005年 The BiP Cochaperone ERdj4 Is Required for B Cell Development and Function ERdj4 is a BiP cochaperone regulated by the unfolded protein response to facilitate degradation of unfolded and/or misfolded proteins in the endoplasmic ...
Attributes (CQAs) are within specification following processing, storage and shipping. Besides sterility, ensuring removal of impurities and endotoxins and preventing Drug Product (DP) degradation is essential in maintaining safety and efficacy during the manufacture and long-term storage of a...
Degradation of the D1 protein of the Photosystem II (PS II) complex was studied in the Fad6/desA::Km r mutant of a cyanobacterium Synechocystis sp. PCC 6... E Kanervo,N Murata,EM Aro - 《Photosynthesis Research》 被引量: 58发表: 1998年 Pigment-protein complexes from the photosynthetic...
coli, which can produce misfolded or inactive proteins18, and the lack of pyroglutamic acid19 at the N-terminus of the CD, because of the biotin carboxyl carrier protein (BCCP) tag, may contribute to this lack of apparent activity. However, mass spectrometry results show the expression of ...
2). The presence of substrate in a subset of the complexes was attributed to either a co-purified endogenous protein substrate or self-degradation product and is consistent with the presence of endogenous substrate observed previously in cryo-EM studies of numerous other AAA+ proteins21,34,35,37...
Urokinase is an enzyme protein isolated from the urine of healthy people or obtained from human kidney tissue culture. It is composed of protein with molecular weight of 33000 mg and 54000 mg, respectively. This product direct effects on endogenous fibrinolysis system, which can catalytic cracking ...