doi:10.1007/BF02895083H BranáFolia MicrobiologicaBraná, H.: Degradation of 5′adenosine monophosphate in a cell-free system of Escherichia coli . Fol. microbiol. 12: 1, 1967.
[17]. we speculate that this was related to the tissue source we chose and the evaluation of a variety of phenotypic markers. in fact, tissue origin is an important determinant of the effect of decellularized matrix degradation products on macrophage polarization. dzikiet al.’s study showed ...
asparagine-linked (N-linked) CFGpS system and a new membrane-dependent CFGpS system based on serine/threonine-linked (O-linked) glycosylation. By applying our optimized methods to increase concentrations of vesicle-bound glycosylation machinery, we shorten the time associated with extract preparation, ...
Relative distribution width, relative to the width after step calibration fit, for all parameters grouped per category:atranscription parameters (KTX),btranslation parameters (KTL),cregulation parameters (Kreg) andddegradation parameters (Kdeg). Full size image Discussion Cell-free synthetic biology has...
Preliminary results are presented on a cell-free system extracted from mycelium of Sclerotinia trifoliorum, which caused the disappearance of maackiain from solution. Activity depended on prior exposure of the mycelium to maackiain and on the inclusion of a protease inhibitor in the extraction buffer...
The effect of metabolic regulators on carbohydrate degradation, which supplies electrons for nitrogen fixation in heterocysts, was investigated. Measurements of nitrogenase and enzyme activities in a cell-free system showed that (1) phospho enol pyruvate interfered with fructose 1,6-bisphosphate aldolase...
Nevertheless, whole cell approaches also bear disadvantages like intracellular degradation reactions, transport limitations as well as low substrate solubility. In this work the hydantoinase and carbamoylase from Arthrobacter crystallopoietes DSM 20117 were investigated with respect to their applicability in...
the body fluid; (vii) the changes that cfDNA molecules undergo after exiting cells (e.g., enzymatic degradation and interaction with other extracellular components); (viii) the stability/half-life of cfDNA in the fluid (e.g., rate of degradation, clearance, or binding and reuptake by cells...
Multimodal epigenetic characterization of cell-free DNA (cfDNA) could improve the performance of blood-based early cancer detection. However, integrative profiling of cfDNA methylome and fragmentome has been technologically challenging. Here, we adapt an
The modularity of the bacterial consortium enables the incorporation of additional strains in our system as a plug-and-play feature. By exploiting this feature, we could confer a new function to our cell-free system, such as expressing proteins from linear templates. To implement this, we added...