Given that different cell death modalities may be dysregulated in different diseases, it is becoming increasingly clear that such assays need to not only quantify the extent of cell death, but they must also be able to distinguish between the various pathways. Here, we systematically describe ...
Dysregulated cell death occurs in many human diseases, and modulating the associated pathways has proved effective in the treatment of cancer, stroke and neurodegenerative disorders. In their review, Kepp and colleagues provide an overview of assays capa
Areas covered: Herein, the authors describe cell-based assays used to discover drugs with the potential to slow neurodegeneration, and their associated disease models. They focus on microscopy technologies that can be adapted to a high-throughput screening format that both detect cell death and ...
Cell death assays for drug discovery 来自 掌桥科研 喜欢 0 阅读量: 124 作者: O Kepp,L Galluzzi,M Lipinski,J Yuan,G Kroemer 展开 摘要: Cell death has an important role in many human diseases, and strategies aimed at modulating the associated pathways have been successfully applied to ...
SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death Yue Y, Nabar N, Shi C, Kamenyeva O, Xiao X, et. al. Cell Death & Disease 2018 vol: 9 (9) pp: 904 Detection of Circulating Tumor Cells in Fluorescence Microscopy Images Based on ANN Classifier Tsuji...
autophagy has a close relationship with programmed cell death, while uncontrolled autophagy itself often induces autophagic cell death in tumor cells. autophagic cell death was originally defined as cell death accompanied by large-scale autophagic vacuolization of the cytoplasm. however, autophagy is a ...
In addition, the revolutionary strategy of immunotherapy, focusing on immune checkpoint blockade (ICB) immunoregulatory pathways such as programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) signaling axis, has achieved effective treatment responses in a portion of clinical cases [33,34...
Adenosine monophosphate-activated protein kinase (AMPK) is associated with the development of liver hepatocellular carcinoma (LIHC). AMPKα2, an α2 subunit of AMPK, is encoded by PRKAA2, and functions as the catalytic core of AMPK. However, the role of
The development of reliable, accessible, and scalable viability assays based on cell death–associated effects of biomarkers is extremely important for efficient screening of drug toxicity. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay is currently one of the ...
Cell death assays for drug discovery. Nat Rev Drug Discov 2011; 10: 221–237. Article CAS Google Scholar Galluzzi L, Zamzami N, de La Motte Rouge T, Lemaire C, Brenner C, Kroemer G . Methods for the assessment of mitochondrial membrane permeabilization in apoptosis. Apoptosis 2007; 12: ...