Introduction Positioning Cellular AssaysImpact on Drug DiscoveryClassification of Cellular AssaysProgress in Tools and Technologies for Cellular Compound ProfilingMembrane Proteins and Fast Cellular Responses Receptors FLIPR Technology for Detection of Intracellular Calcium ReleaseCompetitive Immunoassay for Detection ...
Nature Reviews Drug Discovery volume 10, pages 221–237 (2011)Cite this article 27k Accesses 440 Citations 10 Altmetric Metrics details Key Points In response to cytotoxic insults, cells die by different subroutines that are mainly based on apoptosis and necrosis and often preceded by a global ...
Cell death assays for drug discovery 来自 Springer 喜欢 0 阅读量: 129 作者:O Kepp,L Galluzzi,M Lipinski,J Yuan,G Kroemer 摘要: Cell death has an important role in many human diseases, and strategies aimed at modulating the associated pathways have been successfully applied to treat various ...
These assays can be readily applied to any kinase and are valuable in advancing a program through the early stages of drug discovery. 展开 关键词: HTRF Kinase assay Kinetics Inhibitor Mechanism DOI: 10.1016/j.ab.2006.05.006 被引量: 78 ...
This article discusses some of the in vitro and in vivo assays employed by researchers during the drug discovery and developability assessment phases of the discovery and development processes.Keywords:in vivo assays;in vitro assays;drug discovery;drug development 展开 关键词: discovery lead ...
Practical Applications of XY-69 in Drug Discovery Screening Their research led to the development of a high-throughput assay capable of capturing both orthosteric and allosteric inhibitors. The newly developed assay focused on PLC-γ isozymes, which have several regulatory domains unlike other PLC sub...
Ion channels are well characterised drug targets. However, the techniques used to study ion channel pharmacology have not been particularly applicable to modern drug discovery. The aim of this thesis was to examine the usefulness of both established and novel methods used in the discovery and pharma...
Conventional drug sensitivity assays used to screen prospective anti-cancer agents for cytotoxicity monitor biological processes associated with active growth and proliferation, used as proxies of cell viability. However, these assays are unable to disti
Applications of mass spectrometry in early stages of target based drug discovery Target based drug discoveryFunction-based screeningAffinity-based screeningMass spectrometry (MS) has been applied to drug discovery for many years. With the... G Deng,G Sanyal - 《Journal of Pharmaceutical & Biomedical...
Bioanalysis has continued to evolve in response to the needs of the drug discovery and drug development teams, as well as the continuing improvement of the analytical technology that is used for the various assays. This chapter provides an overview of the various assays that are commonly used in...