7. 小鼠肠道组织 肠道组织里,很多老师都会研究DC细胞,但是DC细胞的标志物CD11C,MHCII在肠道巨噬细胞中也会有表达,老师们在检测和数据分析圈门时要注意哦。 8. 小鼠皮肤组织 除了CD11B,F4/80marker外,可添加EPCAM,CD64,CD24等标...
Conventional APCs that express MHC class II (MHCII) and co‐stimulatory molecules include dendritic cells (DCs) and macrophages. Beyond these conventional APCs, immune stimulatory cells have been more recently shown to extend to a class of atypical APCs, composed of mast cells, basophils, and ...
Clinically Relevant Allergen Mixture Induces Robust Immune Response By Increasing CD11c+CD11b+MHCIIhiCD103int Lung Dendritic CellsClinically Relevant Allergen Mixture Induces Robust Immune Response By Increasing CD11c+CD11b+MHCIIhiCD103int Lung Dendritic CellsFemale Balb/c mice were sensitized intranasall...
Gr1 ?/low CD11b ?/low MHCII + myeloid cells boost T cell anti-tumor efficacy Two major barriers in the immunotherapy of breast cancer include tumor-induced immune suppression and the establishment of long-lasting immune responses against the tumor. Recently, we demonstrated in an animal model ...
结果:用 ADH-503 (GB1275)处理后,巨噬细胞的 MHCI、MHCII、CD80 和 CD86 表达水平更高,表明剩余巨噬细胞的抗原呈递特性有所改善。[1] 体内活性 方法:在大鼠和 C57/B6 小鼠中,给予ADH-503 (GB1275) (30,100mg / kg,口服)测试药代动力学 (PK) 和安全性。
Cells were re-suspended in FACS buffer and incubated with Fc blocking antibody for 30 minutes, to prevent binding of nonspecific FcγRIII/II. Cells were then labeled with indicated antibodies (as a cocktail: Anti-CD11b, anti-CD11c, anti-CD45 anti-Gr1 anti-Siglec F and anti-MHCII), for...
CD11c+MHCII+CD64−DC (left) and relative frequencies of DC subsets (right) in the colonic LP ofRag1−/−Cre−orRag1−/−Cre+mice. Data are pooled from two independent experiments with a total of seven mice per group.eExpression of CD103 and CD11b by live CD11c+MHCII+DC ...
Characterization of kidney CD45intCD11bintF4/80+MHCII+CX3CR1+Ly6C- “intermediate mononuclear phagocytic cells” Kidney immune cells play important roles in pathogenesis of many diseases, including ischemia-reperfusion injury (IRI) and transplant rejection. While studying murine kidney T cells, we se...
Following CD11chighMHCII þ DC subsets were sorted and analyzed in a gene expression microarray: (1) CD103 þ CD11b À , (2) CD103 þ CD11b þ , and (3) CD103 À CD11b þ . (b) Transcript heat map of the B640 genes that are at least twofold differentially expressed ...
(a) Flow cytometry analysis of CD11b þ and CD8a þ in CD11c þ and MHC II þ gated DCs in the spleen of naive mice or mice immunized intravenously (i.v.) with CT 24 h before analysis. (b) CD86 expression in CD11b þ or CD8a þ DCs in naive (gray line) or CT-...