Shown are fixed, permeabilized sporozoites labeled with a 1:800 dilution of polyclonal anti-PfTRAP mouse serum (followed by anti-mouse IgG secondary; green channel), fluorescently labeled anti-PfCSP monoclonal antibody 2A10 (red channel, right image) and DAPI nuclear stain (blue channel, right ...
and goat anti-rabbit 647 (ThermoFisher Scientific, A32733; 1:400) for 1 h. Nuclei were stained with DAPI. Hep-2 cells were visualized by CellMask Orange Plasma membrane stain (Thermofisher Scientific, C100455; 1:10,000). Viability assay for glial cells Primary glial cells were infected wi...
and goat anti-rabbit 647 (ThermoFisher Scientific, A32733; 1:400) for 1 h. Nuclei were stained with DAPI. Hep-2 cells were visualized by CellMask Orange Plasma membrane stain (Thermofisher Scientific, C100455; 1:10,000). Viability assay for glial cells Primary glial cells were infected wi...
LIVE/DEAD™ Fixable Blue Invivogen Cat# L34961 Vectashield Mounting Medium with DAPI Vector labs Cat# H-1200-10 Formalin solution, neutral buffered, 10% Sigma Aldrich Cat# HT501320 Penicillin/Streptomycin Sigma Aldrich Cat# P0781 eBioscience™ Intracellular fixation & permeabilisation buffer ...
We also counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) to detect nuclei. Tumor models and treatment 150,000 B16F10 or 500,000 MC38 cells were injected i.d. into the right flank (or 50,000 B16 in left flank for re-challenge experiment). Tumor growth was ...
Cells were then washed twice in FACS buffer before being resuspended in FACS buffer containing DAPI (1:1,000; Invitrogen) to discriminate live and dead cells. CAFs were isolated according to the following cell surface markers: CD140a+/GP38+/EpCAM−/DAPI−. Following CAF isolation, CAF ...
(1:400, Invitrogen, #A11008. RRID: AB_2534072) with excitation and emission wavelengths of 495/525 nm and 578/603 nm respectively. DAPI staining (1:25,000, Thermo Fisher, #62247, excitation 360 nm, emission 460 nm) was performed to visualize nucleated cells. The slides were mounted ...
Fine, widely spaced dsDNA strands are labeled with DAPI and appear blue in this image, whereas non-typeable H. influenzae are labeled with FITC-conjugated antiserum directed at a surface-exposed protein and appear green. Marker bar = 5 m. (b) DNABII family members have a stronger preference...
48 h after spinfection, transduced cells were selected with 1 mg/mL hygromycin for 10 days. The hygromycin-resistant Ba/F3 luciferase cell line was sorted in the presence of propidium iodide stain to one-cell-per-well into 96-well plates with BD FACSAria Fusion flow cytometer (BD). The ...
19F MRI detected 1.31 ± 0.2 × 105cells (Fig.3a, yellow arrow) in the draining popliteal lymph node. Following MR imaging, CFSE+human19F-PFC-labeled PBMC were detected in cryosections of the same popliteal lymph node (counterstained with DAPI; Fig.3b, green arrows). In a ...