DAPI (Sigma-Aldrich Ltd., St Louis, MI, USA) was added as a viability stain before flow cytometric sorting. One hundred single live cells (DAPI positive cells excluded) for each clonal culture being analysed at P1 were sorted into PCR tubes containing lysis buffer, oligo-dT primer and dNTP...
Fol- lowing treatment with C-1311 for up to 120 h, cells (approxi- mately 2×105) were spun onto microscopic slides, fixed in methanol:acetic acid (3:1) for 15 min and stained with 1 μg/mL DAPI for 5 min. Twenty random fields containing at least 50 cells were examined at 200×...
Each image of each patient depicts the same field of view of the same section, sequentially stained with the fluorescence-labeled antibodies indicated and the nuclear stain DAPI. Magenta arrows indicate IgA2+IgA+CD27+CD38+ cells (containing a nucleus). Images contain 2048 × 2048 pixels ...
LYVE1+ lymphatic endothelial cells (white) and nuclei (DAPI; blue) were visualized in a cross-section of the lymph node. D: Quantification of area coverage of LYVE1+ cells in the DCLN. One experiment was performed. B and D: Statistical significance was measured using t-test. ∗P < ...
Neutrophils are important effector cells of the innate immune system, traditionally regarded to have a short life span. The goal of this study was to evaluate the effect of the whole blood storage on neutrophil functions, e.g., viability, antimicrobial effect, neutrophil extracellular trap (NET)...
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