Trypan blue is a simple-to-use blue dye that cannot pass through the plasma membrane of healthy, live cells; hence it is most often used in cell counting to stain dead cells and in determining cell viability in mouse tissue. H&E staining is a well-known histopathology stain, in which ...
DAPI (4’,6-二脒基-2-苯基吲哚) 是一种常见的细胞核和染色体复染剂,在与 DNA AT 区结合时发出蓝色荧光。 此方案可以用于: 荧光显微镜检测中的核酸(细胞核)染色 此方案不能用 于: 流式细胞仪 完成此方案需要以下材料: 在培养物中生长的细胞
Nuclear stain for HCA/HCS cell demarcation A popular nuclear and chromosome counterstain, DAPI (4′,6-diamidino-2-phenylindole) emits blue fluorescence upon binding to AT regions of DNA. This protocol produces sufficient stain for one 96-well plate at a staining volume of 100 μL/...
Loring, in Human Stem Cell Manual, 2007 DAPI staining This is a simple method used to stain DNA and count chromosomes, but it will not allow identification of individual chromosomes. 1. Add a drop of mounting medium containing DAPI to the slide. 2. Seal the coverslip. 3. Count chromosomes...
1. DAPI dye is more sensitive to staining mammalian cells than staining bacteria. It is recommended to stain with a final concentration of 10 μg / mL in PBS or 150 mM NaCl for 30 min at room temperature to stain bacteria. Dead cells are usually stained more brightly than live cells. ...
Lipid Stain, HCS LipidTOX Red Phospholipidosis Detection Reagent, HCS NuclearMask Blue, HiLyte Fluor 488, HmGFP, Hoechst® 33258, Hoechst® 33342, Kaede Green, Katushka2S, LIVE/DEAD® Fixable Green Dead Cell Stain, LIVE/DEAD® Fixable Red Dead Cell Stain, LysoTracke...
(pH 9), ATTO 390, Cascade Blue®, CellTrace Calcein Violet, CF405M, CF405S, Coumarin 1, Coumarin 30, EBFP, Hoechst® 33258, LIVE/DEAD® Fixable Violet Dead Cell Stain, LysoSensor Blue, LysoTracker® Blue, Marina Blue®, Me-Coumarin, Monobromobimane, Pacific Blue...
It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be...
It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be...
where its blue fluorescence vividly contrasts with red, yellow, or green fluorescent dyes used to stain other structures. It has also been used for studying apoptosis (at low concentrations the dye is excluded from live cells but penetrates dead or damaged cells), and in quantitative DNA assays...