DAPI live cell staining DAPI has the benefit of staining both live and fixed cells. In order to stain live cells, though, the concentration of DAPI must be increased. Common DAPI live cell staining protocols generally involve incubating cells with DAPI stain for approximately 10 minutes, then vi...
DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function. For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, ...
DAPI染色的另外一个替代方案是来自于 Molecular Devices的EarlyTox™ Live Red 染料。该膜可透红色染料可对实验中所有的细胞的细胞核DNA进行标记,无论细胞的活力状态。因此可用于替代DAPI进行成像检测中的核染色。Live Red染料由622 nm 激发,645 nm发射并在使用中不影响FITC 通道。 本应用中我们通过细胞计数实验对...
DAPI is generally used to stain fixed cells since the dye is cell impermeant, although the stain will enter live cells when used at higher concentrations. For live-cell staining, Hoechst 33342 dye is a popular cell-permeant nuclear counterstain. DAPI dashboard Initial brightness DAPI serves as ...
2. Otto F. 1990. DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. Methods in Cell Biology 33: 105-110. 3. Kapuscinski J. 1995. DAPI: a DNA-specific fluorescent probe. Biotechnic & Histochemistry 70(5): ...
The smallest and most delicate species, Balanion planctonicum, was difficult to sort unstained and appeared not to grow after single cell sorting. DAPI-staining at concentrations ranging from 0.1 to 5 mug DAPI ml(-1) improved flow cytometric discrimination between the ciliates and non-target ...
DAPI是一种能够与DNA强力结合的荧光染料。它结合到双链DNA小沟的AT碱基对处，一个DAPI分子可以占据三个碱基对的位置。结合到双链DNA上DAPI 分子的荧光强度提高大约20倍，常用与荧光显微镜观测，根据荧光的强度可以确定DNA的量。另外，因为DAPI可以透过完整的细胞膜，它可以用于活细胞和固定细胞的染色。在荧光显微镜观察...
View and buy high quality DAPI. Blue-fluorescent DNA stain / dye. Used for: nuclear counterstain, fixed and live-cell staining, assessing apoptosis. Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), ISH. Cited in 14 publica
Cell cycle deregulation is associated to several human pathologies, including cancer. The authors developed a method of image analysis based on DAPI-staining which allows the identification of the cell cycle momentum of individual and population of cells. Overall, the imaging framework exhibited a sens...
碧云天研发生产的Beyo3D™ DAPI染色液(Beyo3D™ DAPI Staining Solution),也称3D细胞核染色液(3D Cell Nuclear Staining Solution),是一种快速、便捷的用于3D培养的细胞球或类器官等的细胞核染色的溶液。仅需染色10分钟就可在荧光显微镜下观察到非常明亮的细胞核蓝色荧光染色。如果细胞球或类器官中有凋亡细胞,其...