For more information, Boster offers a simple protocol for staining fixed cells, tissues, adherent, and suspension cells using our DAPI stain. DAPI as a counterstain Due to DAPI’s vibrant blue fluorescence and relatively low background ‘noise’ in labeling, it is frequently used as a ...
The impact of DAPI-staining on the growth of the sorted cells differed between species. However, three of the four species tested grew reasonably well after staining with 0.1 mug DAPI ml(-1). We expect that this staining and sorting protocol may greatly facilitate and improve future experiments...
Staining of Live Cells for Viability Analysis by Flow Cytometry 1.Obtain a single cell suspension. 2.Resuspend cells in BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or 1× Dulbecco's Phosphate Buffered Saline (DPBS) containing 0.05-0.2 μg/mL DAPI. ...
This protocol produces sufficient stain for one 96-well plate at a staining volume of 100 μL/well. This protocol can be used for: Nuclear demarcation in high-content analysis/screening (HCA/HCS) This protocol should not be used for: Flow cytometry You will need the following for th...
This protocol produces sufficient stain for one 96-well plate at a staining volume of 100 μL/well. This protocol can be used for: Nuclear demarcation in high-content analysis/screening (HCA/HCS) This protocol should not be used for: Flow cytometry You will need the following for...
Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One ...
DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound ...
Please adjust the concentration of DAPI working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells(6-well plate) a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is ...
Cell Fixation and Staining Cells were washed with PBS (NaCl 137 mM, KCl 2.7 mM, Na2HPO4 10 mM, KH2PO4 7.4 mM, pH 7.4) and then fixed with 4% formaldehyde/PBS (freshly made) for 15 min. Cells were then quenched for 10 min with 10 mM NH4Cl (PBS) and permeabilized for 10 min ...
Combination of live and fixed cell stains in HeLa cells imaged using the EVOS® FL Auto imaging system Peroxisomes in Caki cells imaged on EVOS® FL Auto imaging system Combination of live Golgi and fixed cell cytoskeletal staining of HeLa cells imaged using the EVOS® FL Auto imaging syst...