它跟DAPI一样也不 会干扰绿色荧光成像,而DAPI已经被证过了。StainFree技术或活细胞红色染料更适用于基于活细胞的实验,并且能够更好地证明实验结果。 参考文献 https://www.lifetechnologies.com/order/catalog/product/D1306 Otto F. 1990. DAPI staining of fixed cells for high-resolution flow cytometry of nucle...
它跟DAPI一样也不会干扰绿色荧光成像,而DAPI已经被证明过了。StainFree技术或活细胞红色染料更适用于基于活细胞的实验,并且能够更好地证明实验结果。 “ 引用文献 1. https://www.lifetechnologies.com/order/catalog/product/D1306 2. Otto F. 1990. DAPI sta...
DAPI live cell staining DAPI has the benefit of staining both live and fixed cells. In order to stain live cells, though, the concentration of DAPI must be increased. Common DAPI live cell staining protocols generally involve incubating cells with DAPI stain for approximately 10 minutes, then vi...
对于在透射光通道中成像的细胞,使用StainFree技术来识别细胞。软件中的预定义设置“CellsA”为这些CHO细胞实现了最精确的结果。StainFree细胞计数与荧光细胞核计数非常接近(图2,图),表明MiniMax细胞计数仪和SoftMax Pro软件可准确消除对细胞核染色计数的需求。使用ImageXpress Micro XLSsystem和MetaXpress®软件对DAPI染色...
StainFree 细胞计数是最大限度地提高细胞活性和减少细胞处理时间的好选择,因为它既不需要荧光染料,也不需要固定。对于需要细胞核染料的情况,例如,为了监测细胞核大小或染色水平,可以使用活的红色染料代替 DAPI,但不需要固定。它也不会干扰绿色荧光成像,因为 DAPI 已被证明是如此。使用 StainFree 技术或 Live RedDye ...
DAPI Nucleic Acid Stain 常见问题解答 (FAQ) We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains...
BPAE cells fixed and permeabilized using the Invitrogen Image-iT Fixation/Permeabilization Kit. Nuclei were stained using Invitrogen NucBlue Live Cell Stain. Alternatives to DAPI We offer a number of nucleic acid stains—with various emission wavelengths—that can be used as alternatives to DAPI for...
DAPI or 4',6-diamidino-2-phenylindole is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI will pass through an intact cell membrane, it may be used to stain both live and fixed cells. ...
DAPI or 4',6-diamidino-2-phenylindole is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI will pass through an intact cell membrane, it may be used to stain both live and fixed cells. ...