因此,发展将小鼠和人类的大脑特征投射到相同的空间的计算方法,研究物种间的保守结构、功能及其差异对深入理解人脑具有重要意义。 近些年来,单细胞转录组测序技术(Single-cell RNA sequencing, scRNA-seq)尤其是空间转录组(spatial transcriptomics,...
Here, we used single-cell RNA sequencing (scRNA-seq) to decipher the composition and functions of GAMs in murine experimental GL261 gliomas grown in male and female mice. We demonstrate distinct transcriptional programs of microglia (MG), monocytes/macrophages (Mo/MΦ), and CNS BAMs. The ident...
Therefore, we use single cell RNA sequencing (scRNA-seq) to reveal distinct immune microenvironment in primary and brain metastatic lesion of NSCLC.Methods:Eight primary and nine brain metastatic NSCLC tumor samples were obtained through surgical resection. Immune cells (CD45+) were enriched through ...
The resource, which is also provided as an interactive web portal, includes 11.3M human cells from 14 main regions and 30 subregions of the brain (Supplementary Fig.1), while the mouse data include 15M cells. Single-cell RNA sequencing and single-nucleus RNA sequencing data of the brain wer...
We analyzed two distinct single-cell RNA sequencing datasets of lung cancer metastasis to analyze the evolutionary trajectory of brain metastatic tumors. In addition, a systematic comparison of cell-cell interaction between tumor cells and lymphocytes was conducted within primary and brain metastatic ...
先与RNA-seq的数据相结合,首先计算每个细胞亚类中单个核的可及性,将255828个远端cCRE连接到14861个假定的靶基因上,结果是在500kb内总共有1661975个gene-cCRE对。接下来,确定cCRE的亚群,其可及性与假定靶基因的表达呈正相关,因此可以在神经元或非神经元类型中作为推测的增强子(图2F,底部)。总共揭示了265049对...
大规模细胞术(飞行时间细胞术 [CyTOF])和单细胞 RNA-seq (scRNA-seq) 结合基于 VDJ 测序 (VDJ-seq) 的 T细胞克隆型鉴定用于确定 COVID-19 和严重程度-T细胞区室的特异性改变。 免疫衰老保守标志物探究 Comprehensive Profiling of an Aging Immune System Reveals Clonal GZMKCD8T Cells as Conserved ...
of single cell genomics at the Center for Epigenomics at University of California, San Diego. As a postdoctoral fellow in Dr. Bing Ren's laboratory at the Ludwig Institute for Cancer Research he studied brain development and cancer using single cell ATAC-seq and single cell RNA-seq approaches....
Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis 回到文章中来 1.Introduction 文章基于他们之前的GESTALT,which used CRISPR–Cas9 barcode editing for large-scale lineage tracing,开发了一个同时表征转录组和谱系发育关系的方法:aninduciblesystem to introduce barcode edits at lat...
Single nucleus RNA-Seq (sNuc-Seq) profiles RNA from tissues that are preserved or cannot be dissociated, but does not provide the throughput required to analyse many cells from complex tissues. Here, we develop DroNc-Seq, massively parallel sNuc-Seq with droplet technology. We profile 29,543 ...