Binding and cleavage (BINACLE) assay for the functional in vitro detection of tetanus toxin: Applicability as alternative method for the safety testing of ... HA Behrensdorf-Nicol,U Bonifas,KM Hanschmann,... - 《Vaccine》 被引量: 6发表: 2013年 Results of an international transferability study...
In order to assess if the improved binding affinity and off-rate observed on the yeast surface can be repeated after purifying each scFv and reversing the orientation of the binding assay (instead of scFv fused to the yeast surface and binding to soluble PD-L1, PD-L1 is immobilized and allo...
This demonstrates the utility of pY1H assays to map these functional relationships and provides new information about how NF-κB and AP-1 subunits combine to enhance or inhibit targeting of certain promoters. Additionally, we observed the expected differences between the 1-AD and 2-AD assay ...
Antheraea polyphemus ApolPBP X17559* (6E, 11Z) hexadeca-6,11-dien-1-yl acetate 0.64 Vial adsorption assay 0.1 n/a 0.1-10 6.8 29 0.48 AMA competitive fluorescence 2 0-20 2 8.0 64 0.80 Tryptophan fluorescence 2 0-8 n/a 6.8 30 (4E, 9Z) tetradeca-4,9-dien-l-yl acetate 21 Vial a...
6.05.3.5.3Promoter–reporter assay The aboveDNA binding assaysonly indicate that a protein is bound to DNA, but cannot indicate whether this results in functional transactivation (or repression) of the promoter. This may be inferred by determining the mRNA level of the target gene (Section6.05....
The inhibition of the activity of β-secretase (BACE-1) is a potentially important approach for the treatment of Alzheimer disease. To explore the mechanism of inhibition, we describe the use of 46 X-ray crystallographic BACE-1/inhibitor complexes to der
Nevertheless, Notch1lbd exhibits reduced signaling through Delta1 and Jagged1 to the same extent as Notch1 null ES cells in a co-culture reporter assay. Moreover, the binding of Delta1 is reduced to the same extent as in cells that lack Notch1 altogether. This is consistent with in vitro...
There has been a recent shift towards in vivo approaches [4]. In the (iv)Chromatin ImmunoPrecipitation (ChIP)assay, a variation of the ‘pull down’ class of assay [12], the DNA-binding protein of interest is cross-linked to the DNA using formaldehyde. The DNA is then fragmented into sm...
& Li, H. A functional single-molecule binding assay via force spectroscopy. Proc. Natl Acad. Sci. USA 104, 15677–15681 (2007). ADS CAS PubMed Google Scholar Yu, H., Shan, X., Wang, S. & Tao, N. Achieving high spatial resolution surface plasmon resonance microscopy with image ...
In this study, we evaluate a wide range of ligands for their binding affinity towards the RGD-binding integrins αvβ3, αvβ5, αvβ6, αvβ8, α5β1, αIIbβ3, using homogenous ELISA-like solid phase binding assay. Structural and signalling responses of cells are tightly regulated ...