There are several advantages to use of cell-based assays versus those using an isolated protein. First, membrane-bound targets are in a more natural environment and surrounded by accessory proteins that operate more normally than when prepared as membrane fractions. Second, one can visualize, in ...
Cell culture system provides powerful advantages that complementin vivostudies. The homogeneity of the cell populations allows consistent and uniform response, thus permitting sensitive and quantitative assessment both in biochemical and cell biological behaviors. The cell-based assays are amenable to chemica...
The single-step BODIPY FL dye–based assay has several advantages over indirect detection of biotinylated or haptenylated nucleotides. With direct detection procedures, no secondary detection reagents are required; fewer protocol step...
This new assay has several clear advantages over other existing cell-based EPAC assays. Compared to the traditional bead-based Rap-GTP pull-down assay, the immobilized RalGDS-RBD bait in microplate enables assay miniaturization and automation. In fact, the assay can be performed in parallel for ...
(FRET), has permitted the engineering of constructs containing both a donor and acceptor fluorophore, with the spatial proximity of both dictated by construct conformation. Intramolecular FRET-based probes are designed to shift conformation in a reversible manner that is dependent uponligand bindingor ...
or monitoring organelle function. Regardless of the type of cell-based assay being used, it is important to know how many viable cells are remaining at the end of the experiment. There are a variety of assay methods that can be used to estimate the number of viable eukaryotic cells. This ...
Although single-cell sequencing offers significant advantages in the study of atherosclerosis, there are also limitations and areas that require improvement. Firstly, single-cell sequencing technology still has a certain error rate, particularly in detecting low-expression genes, which can result in false...
The method is based on the interaction of cell surfaces with chemically derivatized fibers. A variety of molecules such as lectins, antigens, or antibodies can be covalently coupled to the fibers to provide the requisite binding specificity for the cells. Adsorbed cells are removed by various ...
These results have preliminarily demonstrated the advantages of using HA-MSCs in resistance to ovarian aging. Furthermore, we used eGFP and SPION double labeling methods. Magnetic resonance imaging (MRI) technology was used to study the distribution, colonization, and outcome of stem cells in the ...
DNA nanostructures have many characteristics of structuralprogrammability, size controllability, and diverse functions, which are very promising[85]. Currently, biomaterials that own various applications have been developed, including drugs,miRNA,siRNA[86]. etc. In view of the advantages of DNA nanostruct...