Cellular assays, or stable cell-based assays, can be used in both biomedical research and drug-discovery screening applications to efficiently quantify cytotoxicity, biological activity, biochemical mechanisms and off-target interactions. The advantages of cell-based assays include the facilitation and gene...
There are several advantages to use of cell-based assays versus those using an isolated protein. First, membrane-bound targets are in a more natural environment and surrounded by accessory proteins that operate more normally than when prepared as membrane fractions. Second, one can visualize, in ...
Cell culture system provides powerful advantages that complementin vivostudies. The homogeneity of the cell populations allows consistent and uniform response, thus permitting sensitive and quantitative assessment both in biochemical and cell biological behaviors. The cell-based assays are amenable to chemica...
This new assay has several clear advantages over other existing cell-based EPAC assays. Compared to the traditional bead-based Rap-GTP pull-down assay, the immobilized RalGDS-RBD bait in microplate enables assay miniaturization and automation. In fact, the assay can be performed in parallel for ...
The single-step BODIPY FL dye–based assay has several advantages over indirect detection of biotinylated or haptenylated nucleotides. With direct detection procedures, no secondary detection reagents are required; fewer protocol step...
The main advantages of FET based- biosensing technology is that unlike conventional biodetection methods such as phenotypic/metabolic fingerprinting or enzyme-linked immunosorbent assay (ELISA); it (1) provides real-time detections, (2) is portable; (3) has simple transducing mechanisms; (4) is re...
These strategies offer the advantages of speed, cost-effectiveness, genome coverage, and immediate biological relevance [19, 20]. In an effort to identify human genes involved in regulating activity of ERα, we constructed a high-throughput, cell-based functional screening platform by linking a ...
RNA velocity was calculated by velocyto package based on a previous publication ( http://velocyto.org/) [64]. Briefly, expression matrix of unspliced and spliced mRNA in each sample for both Jin668 and TM-1 were generated with velocyto CLI (v.0.17.17) with default parameters. The output ...
Acta Pharmacol Sin 2007 Oct; 28 (10): 1698–1704 Full-length article Development of a quantitative, cell-based, high-content screening assay for epidermal growth factor receptor modulators1 Jue WANG2, Xin XIE2,3 2The National Center for Drug Screening, Shanghai Institute of Materia Medica, ...
task, we chose to pseudobulk by cell neighborhoods as modeling cell states with partially overlapping cell neighborhoods has advantages over clustering into discrete groups. We sampled cell neighborhoods from developing T cells with productive αβTCR (double positive (DP) to mature single positive CD4...