Cellular communication is an essential feature of multicellular organisms. Binding of ligands to their homologous receptors, which activate specific cell signaling pathways, is a basic type of cellular communication and intimately linked to many degenera
Multiplex immunoassays with acellular antigens are well-established based on solid-phase platforms such as the Luminex® technology. Cell barcoding by amine-reactive fluorescent dyes enables analogous cell-based multiplex assays, but requires multiple
Cytotoxicity assay was performed on HCT116 human colon cells (ATCC, #CCL-247™) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, #G3580) which is a colorimetric method based on MTS (3-(4,5-dimethylthiazol-2-yl)−5-(3-carboxymethoxyphenyl)−2-(4-sulfophenyl...
Therefore, the authors concluded that this CPP might be used for further studies on the identification of heterogenic cancer subpopulations, and that it could possibly advance to a tool for future theranostic image-guided applications [38]. Based on the already mentioned peptide PF14, Künnapuu ...
近日《Applications in Plant Sciences》刊登综述详细介绍了叶绿体简单重复序列(cpSSRs)在植物遗传学中的地位。虽然许多植物学研究实验室正在转向最新一代测序方法,来收集分子遗传学数据,但对于许多基础和应用植物科学研究小组来说,cpSSRs仍然是一种有用的方法。
assay cell proliferation in individual cells or in cell populations. The rate of new DNA synthesis can be based on incorporation of a nucleoside analog such as BrdU or EdU into DNA. Detection of these two thymidine analogs varies widely and has implications on the assay results and m...
Many cell permeabilisation methods to mediate internalisation of various molecules to mammalian or bacterial cells have been developed. However, no size-specific permeability assay suitable for both cell types exists. We report the use of intrinsically b
theirbiological activity, have been applied to calculate the membrane permeability of new drugs[128,232]. Nevertheless, they have some limitations, namely, the dataset can be insufficient to predict the biological activity with a high enough accuracy. These methods are based on a set of ...
for improved T-cell activation in one molecule, including checkpoint inhibition but also targeted co-stimulation. We believe our results will enable the clinical application of DNA nanotechnologies and highlight the potential of DNA-origami-based biomolecular engineering strategies for medical applications....
a cell- and 96-well microplate-based high-throughput screening system for inhibitors of antigen presentation. A panel of DRB1 genes plus DRA*01:01 gene were expressed in HEK293T cells and in 3T3 cells, and their binding with biotinylated known self-antigen peptides was measured by flow cytom...