Here, we report the development of a cell-based assay based on the SmoIP sensor for the direct, quantitative, high throughput assessment of the Smo activation state. Using this system we could first demonstrate that individual cells within a largely homogenous, clonal population activate Smo at ...
Multiplex immunoassays with acellular antigens are well-established based on solid-phase platforms such as the Luminex® technology. Cell barcoding by amine-reactive fluorescent dyes enables analogous cell-based multiplex assays, but requires multiple
Differential gene expression analysis was performed based on the tutorial in Harvard Chan Bioinformatics Core (HBC) (https://hbctraining.github.io/scRNA-seq/lessons/pseudobulk_DESeq2_scrnaseq.html). Briefly, the preprocessed, normalized and clustered scRNA-seq data from Seurat workflow was used to...
Differential expression analysis of tumor cells with different immune reactive scores. A Violin plot showing immune reactive score in each MM (up) and patients were divided into high or low status group (bottom). B Bar chart showing the enrichment of specific pathways, based on the HALLMARK, GO...
The single-step BODIPY FL dye–based assay has several advantages over indirect detection of biotinylated or haptenylated nucleotides. With direct detection procedures, no secondary detection reagents are required; fewer protocol step...
Emerging concepts for immune checkpoint blockade-based combination therapies. Cancer Cell. 2018;33:581–98. Article CAS PubMed PubMed Central Google Scholar Xia L, Liu Y, Wang Y. PD-1/PD-L1 blockade therapy in advanced non-small-cell lung cancer: current status and future directions....
Here, we provide a protocol for the selection of conformation-specific intracellular antibody degraders using a cell-based screening method. We applied this protocol to select antibody-based degraders targeting the active form of the small GTPase RHOB (i.e., RHOB-GTP) using an engineered H2882 ...
Moreover, PRMT1 was an insufficient splicing gene identified by the analysis of RNA-Seq results using the R package SEAA. IGV-based visualization showed a notable increase in the retention of the second intron in PRMT1 mRNA (Fig. 6C). To confirm this intron retention, we designed specific ...
Since the screening assay was based on the inhibition of the MYB-2KR mutant we first investigated if teniposide also inhibits the activity of wt MYB. A comparison of the effect of teniposide on Myb-wt and Myb-2KR showed that the activity of both proteins was inhibited, however, the wt...
based on gene expression data.eSelected feature plots of genes used to classify the subpopulations of luminal cells into luminal progenitor cells, intermediate luminal cells, and mature luminal cells.fHeatmap on the MSigDB analysis based on the average normalized expression values of genes in the ...