In vitro cellular binding and uptake assay using mouse NPC To quantitatively determine the PK parameters for target-specific elimination, an in vitro cell-based assay using mouse NPC was developed, as shown in Fig. 3 and in the Materials and Methods Section. The cell population of interest in...
BradfordMM.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem.1976;72:248–54. GeSX,JungD,YaoR.ShinyGO: a graphical gene-set enrichment tool for animals and plants.Bioinformatics.2020;36:2628–9. Vander...
The hydrophobicity of d(X)TAT peptides can in principle contribute to numerous aspects of the interactions between these CPPs and cellular macromolecules. On their journey to the cytosol of cells, hydrophobicity could in principle alter binding with various cell surface proteins, proteoglycans, and ...
(Oakleyet al., 2002). This assay can be used to detect and screen for compound activity against G‐protein‐coupled receptor (GPCR) targets. The assay is based on the desensitization and association ofGPCRswithβ‐arrestin uponligand binding. When aGPCRis activated, it binds to a cytoplasmic...
One of the leading current trends in technology is the miniaturization of devices to the microscale and nanoscale. The highly advanced approaches are based on biological systems, subjected to bioengineering using chemical, enzymatic and recombinant metho
Live/dead cell countingis used to measure the quantity of both live and dead cells in the sample population. For example, the trypan blue dye exclusion method relies on the principle that only dead or dying cells with damaged membranes take up trypan blue so the number of dead (blue) or ...
In this review, we first introduce the principle and properties of general 2D micropatterning techniques, including photolithography-based micropatterning and soft lithography micropatterning. We then focus on the application of 2D micropatterning techniques in single-cell manipulation, cytoskeleton targeting, ...
shipping and storage. Based on historical data, for a particular assay to be acceptable the mean OD value of the two negative control tissues (corresponding to 100% viability) must be ⩾0.8. The acceptable mean relative tissue viability for the positive control (3-min exposure) is ⩽30%....
Since the screening assay was based on the inhibition of the MYB-2KR mutant we first investigated if teniposide also inhibits the activity of wt MYB. A comparison of the effect of teniposide on Myb-wt and Myb-2KR showed that the activity of both proteins was inhibited, however, the wt...
While our present experiments cannot dissect these mechanisms in detail, our results clearly demonstrate that the assay is in principle able to uncover Smo activation independent of the underlying molecular mechanism, and is thus fit for purpose. Discussion We have presented a novel, FACS based HTS...