Binding assays represent another tool to identify markers that might be used to diagnose or treat diseases. Since peptide libraries contain hundreds of peptide combinations, combining these peptides with cells or proteins can provide information on both structure-activity relationships or targets for ther...
Assays for measuring nucleic acid binding proteins and enzyme activitiesProcesses for measuring DNA or RNA binding proteins, specific nucleic acids, as well as enzyme activities using labeled nucleic acids of labeled protein/peptide molecules are provided.Jeffrey A. Heroux...
Bioanalytical techniques making use of DN A-protein binding are described, with applications to the analysis of proteins, DN A lesions, and DNA-protein interaction. Affinity probes, capillary electrophoresis (CE), and laser induced fluorescence polarization (LIFP) are combined to achieve high ...
Synthetic DNA oligonucleotides, known as aptamers, have been increasingly used for affinity binding assays to proteins, as well as for separation studies and as biosensors. Recent advances have been made in protein–DNA binding assays using capillary electrophoresis, laser-induced fluorescence, ...
The BODIPY dye–labeled nucleotides may be particularly useful for fluorescence polarization–based assays of ATP- or GTP-binding proteins. Figure 17.3.10 Principle of fluorescence-based detection of the diadenosine triphosphate hydrolase act...
Conversely, purified viral proteins can be used to distinguish the specific composition of antibodies that may be present. Binding assays are able to identify the subclass of rabies virus antibodies, for example, IgM and IgG, by using a conjugated anti-subclass Ig antibody as the secondary ...
Nucleic Acid Capture Assays Isolating DNA and RNA Binding Proteins Preparing Single-Stranded DNA Templates Single Stranded Templates for Pyrosequencing Immobilization of Long Biotinylated DNA Fragments Sequence-Specific RNA/DNA Capture Sequence-specific DNA...
Biotherapeutic:A class of therapeutic macromolecular compounds, most often proteins and peptides, produced using biotechnology such as recombinant DNA technology. Ligand-binding assays:Analytical methods that frequently use assay reagents with high binding affinity for a target analyte in order to specifica...
The detailed understanding of the binding of small molecules to proteins is the key for the development of novel drugs or to increase the acceptance of substrates by enzymes. Nowadays, computer-aided design of protein–ligand binding is an important tool
The design of proteins that bind to a specific site on the surface of a target protein using no information other than the three-dimensional structure of the target remains a challenge1–5. Here we describe a general solution to this problem that starts