Genomic DNA (gDNA) isolation, plasmid preparation, and DNA gel extraction were performed using nucleotide purification kits purchased from Qiagen or BioBasics. All restriction enzymes, T4 DNA ligase, Quick ligase, Taq DNA polymerase, RNase A, Antarctic phosphatase, and shrimp alkaline phosphatase were...
Genomic DNA was extracted from 0.25 g (fresh weight) homogenized humus soil sample using the PowerSoil DNA isolation kit (Mo Bio Laboratories, Carlsbad, CA, USA), according to the manufacturer’s instructions. The genomic DNA was purified using the Gene Clean Turbo kit (MPBiomedicals, LLC,...
Format/Principle:Spin column (silica membrane) Binding Capacity:≤125 µg DNA/column Operation Time:<60mins Sample Size:5×109bacterial cells, ≤2 ml saliva, ≤2 ml serum or plasma, ≤40 ml urine, ≤1.5 ml Milk, and 1 swab.
Sivakumar, S.M. and Safhi, M.M. (2013) Isolation and screening of bioactive principle from Chaetomorpha antennina against certain bacterial strains. Saudi Pharmaceutical Journal, 21, 119- 121.Sivakumar SM, Mohammed M. Safhi. Isolation and screening of bioactive principle from Chaetomorpha ...
DNA isolation for metagenomic studies Two-milliliter samples, taken from the reactors, were used for total community DNA isolation. The extractions were carried out with a slightly modified version of the Zymo Research kit (D6010, Zymo Research, Irvine, USA). Parallel samples from each reactor ...
These new strategies, based on the analysis of DNA directly extracted from environmental samples, circumvent the steps of isolation and culturing of bacteria, which are known for their selectivity leading to a non-representative view of the extent of bacterial diversity. This review provides an ...
Genomic DNA from kefir cultures was extracted using a PowerSoil® DNA Isolation Kit (MoBio Laboratories, Loker Avenue West Carlsbad, CA, USA), according to the manufacturer’s instructions. Total DNA was sent to DNA Services (DNAS) Facility, at the Research Resources Center, the University of...
While in the last century, the characterization of Salmonella as a species was based on essential phenotypic characteristics and geographic isolation, the application of DNA/DNA hybridization criterion has led to the suppression of all species of Salmonella that are pathogenic to animal...
DNA extraction The total genomic DNA was extracted from the soil samples using the E.N.Z.A. Soil DNA Kit (Omega Bio-tek), following the manufacturer’s protocol (http://omegabiotek.com/store/product/soil-dna-kit/). DNA quantity and quality were evaluated by spectrophotometry (NanoDrop) and...
Genomic DNA (gDNA) isolation, plasmid preparation, and DNA gel extraction were performed using nucleotide purification kits purchased from Qiagen or BioBasics. All restriction enzymes, T4 DNA ligase, Quick ligase, Taq DNA polymerase, RNase A, Antarctic phosphatase, and shrimp alkaline phos- phatase...