Methods Our research complies with all relevant ethical regulations and guidelines. All zebrafish procedures were performed in accordance with the UK Animals (Scientific Procedures) Act with appropriate Home Office Project and Personal animal licences and with local Ethics Committee approval. The studies w...
3i, j). Expectedly, the frequency and the number of pre-HSC IIminus (pre-HSC II without pre-HSC IIplus, representing mature hematopoietic cells) was decreased more dramatically (Supplementary Fig. 3o), demonstrating that Atg5 deletion promotes pre-HSC emergence and blocks their maturation. ...
Respiratory function was determined by oxygen flux rates (pmol s−1 ml) minus background rates and corrected to fibre mass (pmol s−1 ml mg−1). ROS-emission was calculated by taking the rate of ROS-emission (pmol s−1) and correcting it by the weight (mg) of tissue and ...
[148]. Rab7 also regulates the retrograde/MTminus-end-directed transport through the proteins RILP (Rab7-interacting lysosomal protein[149]) and ORPL1 (oxysterol-binding protein-related protein 1 L[150]). The latter protein interacts with AP membrane-bound cholesterol, and in so doing it ...
this study were housed under a 12-hour light/12-hour dark cycle in an animal facility under specific pathogen-free conditions, and maintained at 25 °C with humidity levels between 40% to 70%. In vivo and ex vivo fluorescent imaging in tumor-bearing mice ...
Figure 2Effects of Fanlian Huazhuo Formula group on symptoms associated with nonalcoholic fatty liver disease mice.A: Flow chart of animal experiment protocol; B: Body weight of mice once a week for 10 weeks (n= 8); C: Weight change (pre-treatment weight minus post-treatment weight) of ...
(WT) animals. The number of RGCs was calculated by subtracting from the amount of γ-Synuclein positive cells minus the displaced amacrine double stained γ-Synuclein and Calbindin cells. The control eyes are represented as baseline value,n = 4 animals per group.#p < 0.01 in ...
Cells were cultured in Neurobasal-A Medium Minus Phenol Red (Gibco, 12349-015) supplemented with 1X B-27 Serum-Free Supplement (50X) (Gibco, 17504-044), 2mM GlutaMAX(Gibco), sodium pyruvate (Gibco), and 1% penicillin-streptomycin at 37°C in a humidified incubator with 5% CO2. One-...
of SIN minus-strand RNA levels (s) and HSV-1 immediate early gene ICP27 mRNA levels (t) of triplicate samples at indicated time point, respectively. u, v, Quantitative real-time PCR analyses of relative mRNA levels of interferon beta (IFN-β; u) and the interferon-stimulated gene (ISG)...
ASW1736 cells were treated with the indicated concentrations of GANT61 for 48 h or with 10 μM GANT61 for the indicated lengths of time.B,DSW1736 cells were incubated in the absence or presence of GANT61 (10 μM) minus or plus 5Z (1 or 5 μM) (B) or CQ (5 or 10 ...