In simple terms, T24 and EJ cells were lysed in RIP lysates and incubated with magnetic beads containing antibodies specifically recognizing VCP proteins. IgG was used as a negative control. RNA samples bound to the magnetic beads were eluted as RT templates, and the relative content of circHIP...
The Science: Autophagy What is it? Autophagy is the removal of damaged components within the cell to maintain and improve health. How does it work? Autophagy creates autophagosomes that capture and degrade damaged cellular components. Why is it important? Autophagy is a quality control system that...
Common regulatory pathways in both ferroptosis and autophagy Even though strong links exist between ferroptosis and autophagy, the relationship between them is more than a simple positive or negative correlation, different regulatory mechanisms emerge depending on the situation. Therefore, finding the common...
Ali et al. Molecular Medicine (2023) 29:142 https://doi.org/10.1186/s10020-023-00742-2 Molecular Medicine REVIEW Autophagy and autophagy signaling in Epilepsy: possible role of autophagy activator Open Access Naif H. Ali1†, Hayder M. Al-kuraishy2†, Ali I. Al-Gareeb2, Saud ...
In terms of the mechanism, we demonstrated that SIRT4 activated the phosphorylation of p53 protein by suppressing glutamine metabolism, which was crucial in SIRT4-induced autophagy. AMPKα was implicated in the regulation of autophagy and phosphorylation of p53 mediated by SIRT4, contributing to the...
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Here, we provide a detailed description of a simple assay for quantitatively monitoring glycolysis and mitochondrial respiration in cultured cancer cells that mount an adaptive autophagic response to stress. In this setting,flux analysisis employed to measure extracellular acidification rate (ECAR) as an...
Indeed, intracytoplasmic amyloidogenesis might, in specific ways, be a component system in production of both synapse loss and neuronal cell death largely in terms of the etiologic cause of the amyloidogenesis as a prototype of cellular degeneration in its own right. It might perhaps be relevant...
Autophagy is like a built-in cellular mechanic. Within cells, autophagosomes do the work of shuttling problematic parts to lysosomes, which then break-down and digest the bad parts and recycle elements that can be repurposed. Essentially, your cells eat their parts to promote their survival and...
Autophagy is the major intracellular degradation system by which cytoplasmic materials are delivered to and degraded in the lysosome. However, the purpose of autophagy is not the simple elimination of materials, but instead, autophagy serves as a dynamic recycling system that produces new building ...