MEFs omitting biotin (–Bio) were used as a negative control. Molecular weights are indicated. k, TMRE signal intensity distribution of mitochondria isolated from MEFs expressing P5CS–GFP. GFP signal was used to distinguish mitochondria containing P5CS (P5CS–GFP+) from mitochondria lacking P5...
Full size table The current structures show the highest resolution (1.78–2.29 Å) among all ALDH1A3 structures deposited in the PDB (Supplementary Table1). All crystal structures contain a homodimer in the asymmetric unit (Fig.1a, left). The relevantly biological assembly of the enzyme, ...
Eckle T, Fullbier L, Wehrmann M, Khoury J, Mittelbronn M, Ibla J, Rosenberger P, Eltzschig HK (2007a) Identification of ectonucleotidases CD39 and CD73 in innate protection during acute lung injury. J Immunol 178:8127–8137 PubMedCASGoogle Scholar Eckle T, Krahn T, Grenz A, Kohler ...
2d). Taken together, these results suggest that F-ATPase and VGCC can form a protein complex on the plasma membrane of neutrophils. Table 1 Peptides of VGCCA2D1 identified by BN-PAGE Full size table Fig. 2 F-ATPase/VGCC protein complex verification. a. 1D immunoblot analysis of the ...
Download: Download full-size image Fig. 6. Comparison of the fluorescence intensities of the sensing system upon HCR in different ATP solutions using different MEF substrates (AuNBPs and AuNRs) and using no MEF substrate. 3.8. Specificity of SATMEF We also investigated the selectivity of this ME...
Download: Download full-size image FIGURE 7.. Caspase-insensitive ATP release stimulated by chemotherapeutic drugs is resistant to carbenoxolone blockade but suppressed by intracellular Ca2+ buffering. A, schematic of alternative ATP release pathways. When caspases are inhibited, ATP may be secreted ...
Thus, three genes ancestral to full transporters expanded to form groups1-4, containing 2, 8, 9 and 10 genes, respectively (Figure 2b). Similar phenomena also were observed in the ABCB (Figure 3) and ABCC families (data not shown). The ABCG family differed from the others in being ...
In T. japonicus, we identified 17 ABCC transporters consisting of 16 full transporters (two TMDs and two NBDs) and one short form transporter (two TMDs and one NBD) (Figure 2, Additional file 4, Table 1). The D. pulex and D. melanogaster ABCC subfamilies contained seven full transporters...
By making the DNA semi-opaque, the thirteen histone-DNA contacts can be visualized (flashing white, and then staying blue) to form a positively-charged staircase along the surface of the octamer, upon which the negatively-charged DNA is wrapped. By unwrapping the DNA, the canonical octamer is...
Full size image ATP7A functions to limit lysosomal degradation of VEGFR2 in VEGF-stimulated ECs A cell surface biotinylation assay revealed that VEGF stimulation increased cell surface ATP7A levels within 5 min, followed by a gradual decrease over the next 15 min, which was also associated wi...