European Journal of BiochemistryFeldman, R.J. and Sigman, D.S., Eur. J. Biochem. , 1984, vol. 143, no. 3, p. 583.Feldman, R. I. and D. S. Sigman (1984) Enzymatic activities in thylakoid membranes which form medium (3ZP)NDPand ("P) ATP from 3zPi. Eur. J. Biochem. 143,...
In addition to acting as a cellular energy source, ATP can also act as a damage-associated molecular pattern in both animals and plants. Stomata are leaf pores that control gas exchange and, therefore, impact critical functions such as photosynthesis, dr
Full size image We next determined crystal structures with osimertinib and the allosteric inhibitor JBJ-125, which unlike EAI045 synergizes with osimertinib in cells22. The P-loop dramatically folds downward and inwards toward the allosteric inhibitor when in complex with osimertinib and JBJ-125 or ...
Nearly, all cells from very early life‐forms release ATP. ATP release is recognized throughout the phylogenetic tree, being present in bacteria, yeasts, plants, prokaryotic single-celled organisms, and in invertebrates. In mammals ATP release is found in neurons and secretory cells, astrocytes, m...
Full size table How structure relates to function: the rotary F1FoATP synthase The function of ATP synthase is to synthesize ATP from ADP and inorganic phosphate (Pi) in the F1sector. This is possible due to energy derived from a gradient of protons which cross the inner mitochondrial membrane...
The ABCB subfamily is composed of four full transporters and seven half transporters, and this is the only human subfamily to have both types of transporters. The ABCB1 (MDR/PGY1) gene was discovered as a protein overexpressed in certain drug-resistant tumor cell lines. Cells that overexpress ...
A reanalysis of the Pfam database family for DctP-TRAP SBPs (SBP-bac-7) reveals that 5943 of 6142 full-length sequences (96.8%) have an arginine residue in an equivalent position to Arg-147 in SiaP. To investigate the involvement of SiaP residue Arg-147 in transporter function, we mutat...
Full size table Thermal transitions in rigor muscle Earlier measurements performed on myosin showed that at least five endothermic transitions could be observed on bovine heart myosin. The peak maxima were at 17.5, 41.5, 45, 48 and 54.5°C; the estimated transition enthalpies were 150 kcal/mol,...
In eukaryotes, ABC transporters that utilize the energy of ATP hydrolysis to expel cellular substrates into the environment are responsible for most of the efflux from cells. Many members of the superfamily of ABC transporters have been linked with resis
Department of Biochemistry and Molecular Genetics, University of Illinois College of Medicine, Chicago, IL, USA Jack H. Kaplan Departments of Pharmacology and Toxicology, Medical College of Georgia at Augusta University, Augusta, GA, USA Tohru Fukai Department of Medicine (Cardiology), Medical College...