不同的药物浓度 处理，然后在12h时，裂解测量luciferase ，发现luciferase 值下降， β-galactosidase也下降...
In an antioxidant response element (ARE)-luciferase reporter assay, afzelin increased ARE-luciferase reporter activity in a concentration-dependent manner. Consistently, the expression of antioxidant genes, including NF-E2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and catalase, was enhanced...
(A) Cytotoxicity of CPT determined by MTS assay in HepG2 cells. Cells were treated with CPT at indicated concentrations for 24 h; n=8. (B) The ARE luciferase activity under control and NaAsO2-treated conditions in HepG2 cells. Cells were exposed to various concentrations of CPT in ...
AREB6-Luc萤光素酶报告基因质粒(AREB6 luciferase reporter plasmid )是翌圣生物自主研发的用于检测AREB6激活水平为目的的报告基因。 AREB6又称ZEB1是一种锌指结构转录因子,它编码的蛋白在IL-2的转录抑制中起重要作用。另外,AREB6还与肿瘤的发生、发展、凋亡和侵袭转移等生理活动密切相关。
Luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI). Mitochondrial-associated membranes fractionation isolation The subcellular fractionation was performed based on published protocols50. HUVECs were harvested and washed with PBS by centrifugation at 600...
We also evaluated the VEGF promoter activity of ERRα in cervical cancer cell lines by transfection and luciferase assay. We overexpressed or knocked down ERRα and examined VEGF expression by real-time polymerase chain reaction. Finally, cell proliferation assay was performed to examine whether ERR...
ARE-Luc-EF1α-mCherry SH-SY5Y Cells,即ARE-Luc-EF1α-mCherry-T2A-Puro SH-SY5Y细胞,是一种细胞基因组中含有多个ARE结合位点的多克隆SH-SY5Y稳定细胞株(Polyclonal SH-SY5Y stable cell line),通过检测萤火虫萤光素酶(Firefly luciferase, Luc)的活性,可以高灵敏度地检测ARE的激活水平。本细胞株可通过EF1α启...
A recombinant baculovirus vAc beta hCG-luc was constructed that carried the cDNAs encoding firefly luciferase (luc) and beta-subunit of human chorionic gonadotropin (beta hCG) placed under the transcriptional control of individual copies of the baculovirus polyhedrin gene promoter. The simple, rapid, ...
Then, 24 h post transfection, cells were stimulated with 10 μM LysoPS for 24 h and collected for measurement of luciferase activity. Chromatin immunoprecipitation Chromatin immunoprecipitation was performed using a CHIP assay Kit (ThermoFisher Scientific, 26156) according to the instructions ...
2 shows representative gating strategies for FACS-based apoptosis assay analysis. Reporting Summary Supplementary Table 1 MST and luciferase data. Combined tables for the raw MST data and the normalized luciferase reporter activity for each construct used in the study. Related to Fig. 2. ...