The basis of a reporter assay is the vector, which carries the reporter gene, and the detection reagents that provide a substrate to convert the activity of the luciferase enzyme into a light signal. Dual-luciferase detection reagents quench the first reporter signal before reading the second to...
Luciferase assay for the functional analysis of HIV Tat and LTR in gene expression[J]. 于小兵,袁建刚,陈菊凤,强伯勤.Progress in Natural Science. 1996(03)于小兵,袁建刚,陈菊凤,强伯勤.Luciferase assay for the functional analysis of HIV Tat and LTR in gene expression[J]. Progress in Natural ...
Luciferase Assay 的详细介绍 Description The Luciferase Assay Kit is designed for the accurate and efficient determination of firefly (P. pyralis) luciferase gene (luc) expression mammalian or bacterial cell cultures. It can be used in either low- or high-throughput assays. Usage This product is ...
the pull-down assay uses a DNA probe labeled with a high affinity tag, such as biotin, which allows the probe to be recovered or immobilized. A biotinylated DNA probe can be complexed with a protein from a cell lysate in a reaction similar to that used in the EMSA and then...
荧光素酶报告系统 luciferase reporter assay design
assay protocol was run in well-mode from the Gen5™ Data Analysis software (BioTek Instruments, Inc., Winooski, VT) with the parameters outlined in Table 1 and data exported to either Microsoft™ Excel...
Quantitative analysis 5 Data organization Total 25 Case Study To examine whether proteins A and B interact, the assay was performed using both in vivo imaging and plate reader techniques for qualitative and quantitative analysis. As shown in Figure 1A, the in vivo imaging reveals strong luminescence...
When data of the firefly luciferase complementation assay are quantitatively analyzed, the detected luminescence is assumed to correlate with the interaction of the protein pair directly and linearly. The figure describes the assumption. N: a protein fused to the N terminal domain of firefly ...
luciferasereporter gene assayhigh‐throughput screeningRNAiLuciferase reporter gene assays are one of the most common methods for monitoring gene activity. Because of their sensitivity, dynamic range, and lack of endogenous activity, luciferase assays have been particularly useful for functional genomics in...
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