There is provided a process of inducing luminescent emission from a luciferase bioluminescent reaction particularly useful in binding assays. A luciferase bioluminescent combination, together with an inactive, caged trigger compound such as a cofactor, is subjected to photonic radiation, so as to release...
Finally, the IL-8 Luc assay combined with DPRA and DEREK could improve substantially its performance. These data suggest that the IL-8 Luc assay is a promising test method to screen skin sensitizers. 展开 关键词: Contact dermatitis Sensitization test Luciferase assay Alternative method Skin ...
Weitere in-vitro Experimente sind notig. In einer anderen Anwendung wurden beide Luciferase Assays in einem dualen Luciferase Assay kombiniert. Dabei wurde eine Co-Transfektion von Firefly und Gaussia Luciferasen durchgefuhrt und die Robustheit beider Assays gegenuber kleineren Anderungen der ...
functional effects ofmiR-29amanipulation on collagen matrix synthesisin vitro. Using luciferase assays, we first confirmed thatmiR-29adirectly targetsCOL 1A1and3A1as previously demonstrated28(Supplementary Fig. 6c). We also observed a previously unrecognized interaction ofmiR-29awith humanCOL 1A2subunit...
QUANTI-Luc™ 4 is a liquid ready-to-use bioluminescent assay reagent to measure the activity ofLucia® luciferaseand other coelenterazine-utilizing luciferases.In order to meet your practical needs, InvivoGen offers: — QUANTI-Luc™ 4 Lucia/Gaussia: for the detection of secreted Lucia® ...
and the calculated ΔGbindingvalues for each compound yielded a correlation R2of 0.68 (Supplementary Fig.4). As additional controls, we obtained previously developed, commercially available Wnt inhibitors and tested them in the Wnt luciferase assay (Supplementary Fig.5). Inhibitors upstream of β-...
ERα/ERα mutant expression was determined to be equivalent per unit protein, and luciferase reporter assay normalized to total protein. Each treatment group was plated in quadruplicate and the experiment repeated a minimum of three times. Quantitative real-time RT-PCR Real time RT-PCR to measure...
A dual luciferase assay kit (Promega) was used for this experiment. The pRL-null vector (Promega) expressing Renilla luciferase was an internal control. In vivo tumorigenesis assay For the in vivo tumorigenesis assay in mice, MGC803 cells were inoculated with the corresponding lentiviral cells. ...
The clear supernatant was further diluted by 20 times with PLB and applied to dual luciferase assay using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s manual. Alignment of the sequencing reads For rG4-seq libraries, raw reads were directly used for ...
Furthermore, in in vitro analyses of cultured hepatocytes, ChIP demonstrated that ATF3 directly bound to the RIPK3 gene promoter region, luciferase assay analysis of RIPK3 promoter activity revealed that ATF3 regulated RIPK3 gene expression and hepatocyte live-cell imaging showed that ATF3 ...