There is provided a process of inducing luminescent emission from a luciferase bioluminescent reaction particularly useful in binding assays. A luciferase bioluminescent combination, together with an inactive, caged trigger compound such as a cofactor, is subjected to photonic radiation, so as to release...
Weitere in-vitro Experimente sind notig. In einer anderen Anwendung wurden beide Luciferase Assays in einem dualen Luciferase Assay kombiniert. Dabei wurde eine Co-Transfektion von Firefly und Gaussia Luciferasen durchgefuhrt und die Robustheit beider Assays gegenuber kleineren Anderungen der ...
Finally, the IL-8 Luc assay combined with DPRA and DEREK could improve substantially its performance. These data suggest that the IL-8 Luc assay is a promising test method to screen skin sensitizers. 展开 关键词: Contact dermatitis Sensitization test Luciferase assay Alternative method Skin ...
QUANTI-Luc™ 4 is a liquid ready-to-use bioluminescent assay reagent to measure the activity ofLucia® luciferaseand other coelenterazine-utilizing luciferases.In order to meet your practical needs, InvivoGen offers: — QUANTI-Luc™ 4 Lucia/Gaussia: for the detection of secreted Lucia® ...
functional effects ofmiR-29amanipulation on collagen matrix synthesisin vitro. Using luciferase assays, we first confirmed thatmiR-29adirectly targetsCOL 1A1and3A1as previously demonstrated28(Supplementary Fig. 6c). We also observed a previously unrecognized interaction ofmiR-29awith humanCOL 1A2subunit...
and the calculated ΔGbindingvalues for each compound yielded a correlation R2of 0.68 (Supplementary Fig.4). As additional controls, we obtained previously developed, commercially available Wnt inhibitors and tested them in the Wnt luciferase assay (Supplementary Fig.5). Inhibitors upstream of β-...
Medicilon's cell viability assay platform can provide compliant in vitro cell viability detection services that meet the application requirements.
e Luciferase assay analysis of the FDX1-luciferase activity after METTL16 overexpression in HGC-27 cells (n = 3). P = 0.0044. f Prediction of m6A modification sites. The secondary structure of high-confidence prediction sites is shown. g The structure chart showing the predicted m6A...
(Fig.1a). To further differentiate the compounds we assayed them in the nanoluciferase degradation assay, which showed more dynamic range than the imaging assay. Of the two compounds,5showed greater potency in the nanoluciferase assay and lower mouse hepatic clearance than6(Table1and Fig.1a). ...
Indeed, SR1 effectively inhibited Kyn-induced SLC7A8 upregulation in MEPs both in vitro and in vivo (Fig. 4i and Extended Data Fig. 4k). A CUT&RUN assay also showed that AhR bound to the Slc7a8 promoter (Fig. 4j), and a luciferase assay verified that AhR promoted Slc7a8 expression...