5.Mix 20µl of cell lysate with 100µl of Luciferase Assay Reagent and measure the light produced.Preparation of Plant Tissue Lysate 1.Quick-freeze the tissue in liquid nitrogen, grind the frozen tissue to a powder and resuspend in room temper-ature 1X lysis reagent with homogenization.2...
Cite this protocol Gage, M.C., Pourcet, B., Pineda-Torra, I. (2016). Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity. In: Waugh, M. (eds) Lipid Signaling Protocols. Methods in Molecular Biology, vol 1376. Humana Press, New York, NY. https://doi.org/10....
Luciferase Assay System (Promega) is used to evaluate luciferase activity. In brief, cells in a 96-well plate are rinsed once with PBS. After removing PBS completely, 1× Reporter Lysis Buffer is dispensed into individual wells (20 μl/well). After freezing and thawing twice and agitation fo...
dual-glo-luciferase-assay-system-protocol 下载积分: 300 内容提示: Revised 9/15 TM058T E C H N I C A L M A N U A LDual-Glo ® Luciferase Assay SystemInstructions for use of Products E2920, E2940 and E2980. 文档格式:PDF | 页数:26 | 浏览次数:29 | 上传日期:2021-03-17 09:...
Including coenzyme A in the reaction enhances the sensitivity of the assay and provides a sustained light reaction. In this protocol, cells transfected with a luciferase reporter plasmid are lysed using a detergent-containing buffer. Cell debris is removed by ...
Reporter assayThe measurement of the transcriptional activity of the HH signaling pathway is widely used as an indication of pathway activation. Luciferase reporter assays are powerful tools to measure the specific ability of a transcription factor to bind to its consensus sequence and to activate ...
4.A. Protocol for Manual Luminometers 1. Dispense 100µl of the Luciferase Assay Reagent into luminometer tubes, one tube per sample. 2. Program the luminometer to perform a 2-second measurement delay followed by a 10-second measurement read for luciferase activity. The read time may be ...
Tissues were frozen directly in liquid ®nitrogen and stored at − 86 °C for 1 day before performing luciferase activity assay – by following the protocol by the manufacturer. The luciferase was detected using a Luminoscan Ascent micro plate illuminometer (Thermo Electron Oy, Vantaa...
simultaneous monitoring of experimental reporter and control luciferase activity in a single-read assay without the need for two-step addition of substrate reagents or quenching (Figure 3). Specificity analysis of the two luciferase emissions has less than 1% of Gaussia light crossover to the 640...
Sensitive: highly sensitive detection of firefly luciferase activity. Stable: the signal is stable for more than two hours, providing flexibility regarding incubation time. Convenient: simple one-step, homogeneous protocol. High-throughput: the one-step protocol minimizes handling steps to support high-...