a我已經測試功能 I already tested the function[translate] aluciferase reporter gene assay luciferase记者基因分析用试样[translate] ai am serious this time 我是严肃的这次[translate] aING Belgium ING比利时[translate] a和开发企业进行协商 Carries on the consultation with the development enterprise[translate]...
a劳动力市场供过于求 Labor market supply in excess of demand[translate] a获得机会参加决赛 正在翻译,请等待...[translate] aFor the luciferase protection assay (Fig. 6), WT and CS3 protoplasts were cotransformed with a plasmid encoding firefly luciferase (pLuc) together with empty pRT vector as...
In early 1990s, direct expression of external mRNA molecules in host animals for therapeutic effects was first tested by delivering RNA vectors encoding a reporter gene such as luciferase and β-galactosidase into murine muscle cells and transfecting vasopressin mRNA into rats to reverse Diabetes-Insip...
2.4.4. NMD Luciferase Reporter Assay In this study, we used a highly sensitive and quantitative NMD dual luciferase reporter system by measuring WT- and PTC-containing β-globin mRNA levels [7]. pCDNA6-RSV-Renilla-Luc-β-globin (WT) (Renilla-WT) is expressed independent of NMD in the cel...
fused Gaussia luciferase, a 19 kDa protein, to the N-terminus of VP2 without affecting titer or transduction efficiency of the resulting vectors. This enabled the tracking of bioluminescent viral shells based on AAV1, AAV2 and AAV8 in vivo [146]. Finally, recent work from the Buchholz lab...
By using the temperate phage Wβ, the luciferase enzyme can accumulate within the bacterial host to increase the amount of bioluminescence produced, thus increasing the sensitivity of the assay. The drawback of using the phages γ and Wβ is that they can infect only vegetative cells and not ...
Overexpression of TP53 increased Pol η mRNA levels and enhanced luciferase activity in a reporter assay. Importantly, overexpression of a mutant TP53 (R175H) was unable to enhance luciferase expression. Unpublished data from our laboratory and other studies have confirmed higher expression levels of ...