a劳动力市场供过于求 Labor market supply in excess of demand[translate] a获得机会参加决赛 正在翻译,请等待...[translate] aFor the luciferase protection assay (Fig. 6), WT and CS3 protoplasts were cotransformed with a plasmid encoding firefly luciferase (pLuc) together with empty pRT vector as...
a我已經測試功能 I already tested the function[translate] aluciferase reporter gene assay luciferase记者基因分析用试样[translate] ai am serious this time 我是严肃的这次[translate] aING Belgium ING比利时[translate] a和开发企业进行协商 Carries on the consultation with the development enterprise[translate]...
In early 1990s, direct expression of external mRNA molecules in host animals for therapeutic effects was first tested by delivering RNA vectors encoding a reporter gene such as luciferase and β-galactosidase into murine muscle cells and transfecting vasopressin mRNA into rats to reverse Diabetes-Insip...
(2008) have monitored AMP/ATP by HPLC and total ATP by luciferase assays in zebrafish. Also, Niethammer et al. (2009) have successfully used a genetically encoded H2O2 sensor in zebrafish. Concluding remarks In summary, the present work supports zebrafish for studying mitochondrial dysfunction ...
Next, we assessed protein expression for each delivery vehicle, as the relationship between nanoparticle biodistribution and protein expression does not always correlate. To do this, we formulated nanoparticles with unmodified firefly luciferase-encoding mRNA (mLuc) and delivered them intravenously at a ...
2.4.4. NMD Luciferase Reporter Assay In this study, we used a highly sensitive and quantitative NMD dual luciferase reporter system by measuring WT- and PTC-containing β-globin mRNA levels [7]. pCDNA6-RSV-Renilla-Luc-β-globin (WT) (Renilla-WT) is expressed independent of NMD in the cel...
Overexpression of TP53 increased Pol η mRNA levels and enhanced luciferase activity in a reporter assay. Importantly, overexpression of a mutant TP53 (R175H) was unable to enhance luciferase expression. Unpublished data from our laboratory and other studies have confirmed higher expression levels of ...