What Information Does a Luciferase Assay Tell you? The luciferase reporter assay allows you to study the regulatory control of a gene of interest. The presence of light means that luciferase was transcribed, and therefore the regulatory region was active. This can allow you to determine if a pa...
In its simplest form, the Rluc-LC3-assay can be used to estimate autophagic flux at a single time point by defining the luciferase activities in cell extracts. Moreover, the use of a live cell luciferase substrate makes it possible to monitor changes in autophagic activity in live cells in ...
which were then verified using RNA pull-down and luciferase reporter assays. A murine model of osteoarthritis, characterized by anterior cruciate ligament transection, was then created to investigate the in vivo implications of these observations. These experiments revealed a significant upsurge in miR-...
Next, we assessed protein expression for each delivery vehicle, as the relationship between nanoparticle biodistribution and protein expression does not always correlate. To do this, we formulated nanoparticles with unmodified firefly luciferase-encoding mRNA (mLuc) and delivered them intravenously at a ...
Infection levels were determined in 2 days with Bright-Glo luciferase assay system (Promega, Madison, WI). Neutralization curves were fitted to a 5-parameter nonlinear regression analysis to obtain IC50, the antibody concentration required to inhibit infection by 50%. Dendrograms depicting the gp160...
What is the Best Assay for Monitoring CAR-T Cell Persistence Real-Time In Vivo? You can track CAR-T persistence real-time by labeling with luciferase or fluorescent proteins (e.g. GFP, etc.). What Sort of Models would be Suitable for CAR-NK Cell Therapies? Similar to CAR-T, models ex...
For example, a study using 14C labeled DLH2 revealed that, after 15 min of intraperitoneal administration, DLH2 is distributed mainly at the skin and does not reach the brain or lungs [62]. Accordingly, numerous luciferin analogues [63,64] and mutant luciferases [65,66,67,68,69,70] ...
For example, a study using 14C labeled DLH2 revealed that, after 15 min of intraperitoneal administration, DLH2 is distributed mainly at the skin and does not reach the brain or lungs [62]. Accordingly, numerous luciferin analogues [63,64] and mutant luciferases [65,66,67,68,69,70] ...
Overexpression of TP53 increased Pol η mRNA levels and enhanced luciferase activity in a reporter assay. Importantly, overexpression of a mutant TP53 (R175H) was unable to enhance luciferase expression. Unpublished data from our laboratory and other studies have confirmed higher expression levels of ...