Related to luciferase:luciferin lu·cif·er·ase (lo͞o-sĭf′ə-rās′, -rāz′) n. An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin and ATP, producing light. [lucifer(in)+-ase.] ...
The present disclosure provides a luciferase reporter system applicable in a liquid medium having oxidized flavins and carboxylic acids as substrates. Particularly, the luciferase-based reporter system comprises a recombinant carboxylic reductase operable to convert one or more carboxylic acids into one or...
The luciferase assay was performed with Dual-Luciferase Reporter Assay System (VazymE) according to the manufacturer’s instructions. Luminescence was detected with SPARK System (TECAN). HCV-IRES Renilla were used as internal controls. MS2bp-GFP RNA pull down MS2-GFP-RIP assays with the CDS ...
vector harboring USP16-WT or USP16 with a mutated promoter region (Mutant) and Nrf2. After 36 h, a luciferase assay was performed. Unexpectedly, overexpression of Nrf2 even upregulated the luciferase reporter expression from USP16-WT, but not from USP16 Mutant (Fig.8F). However, in H/...
The luciferase reporter assay Briefly, the cells were plated in 12-well plates at a density of 1.4 × 105cells per well and then transfected with 0.8 μg of promoter-luciferase plasmid. To normalize the transfection efficiency, the cells were also cotransfected with 8 ng of pRL-CM...
The luciferase reporter assay confirmed that γ-linolenic acid could activate PPARα signaling (Figure S7F). However, we did not find direct activation of PPARα by HDCA (Figure S7G). To investigate the mechanism by which γ-linolenic acid modulates CYP7A1, we examined the transcriptional ...
Measuring Luminescence of the Bright-Glo™ Luciferase Assay System using the GloMax® Discover System Application Note P Corporation 被引量: 0发表: 0年 Measuring Fluorescence Using the ApoTox-Glo™ Triplex Assay With the GloMax® Discover... P Corporation 被引量: 0发表: 0年 ...
7e-f, the luciferase reporter assay revealed that the wild-type 3’UTR of ULK1 showed low expression in the presence of miR-20a-5p, whereas the mutated 3’UTR did not show a significant response to miR-20a-5p. In addition, we tested whether PVT1 affects the activity of miR-20a-5p. ...
In the apoptosis assay, the cells were treated with the appropriate reagents. After that, the cells were stained with propidium iodide and Annexin V. The proportion of each cell phase was determined. Luciferase reporter assay Gastric cancer MGC803 and Hs746T cells were cotransfected and plated ...
Luciferase reporter assay HEK293 MSR cells were seeded into 96-well plates and transfected with Lipofectamine 2000 in accordance with the manufacturer’s instructions. In all, 24 h post-transfection, cells were treated for 8 h with either 50 ng/ml Oncostatin M (Biomol, 97241) or 50 ...