Screening the expression level of the reporter gene using either a qualitative or a quantitative assay, allows to identify the regulatory regions of interest (e.g., cis-acting elements or enhancer) in the promoter. Luciferase genes have been widely used as reporter genes for their sensitivity ...
Tannous BA (2009) Gaussia luciferase reporter assay for monitoring of biological processes in culture and in vivo. Nat Protoc 4(4):582–591 CrossRef Tannous BA, Kim DE, Fernandez JL, Weissleder R, Breakefield XO (2005) Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in...
Nano-Glo® Dual-Luciferase Reporter Assay System Ultra-sensitive detection of firefly and NanoLuc® luciferase activities in a single sample. N1610, N1620, N1630, N1650, N1521, N1531, N1541, N1551 ONE-Glo™ EX Luciferase Assay System ...
The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, and contains a modified coding region for firefly (Photinus pyralis)luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of ...
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