aluciferase reporter gene assay luciferase记者基因分析用试样[translate] ai am serious this time 我是严肃的这次[translate] aING Belgium ING比利时[translate] a和开发企业进行协商 Carries on the consultation with the development enterprise[translate] ...
In early 1990s, direct expression of external mRNA molecules in host animals for therapeutic effects was first tested by delivering RNA vectors encoding a reporter gene such as luciferase and β-galactosidase into murine muscle cells and transfecting vasopressin mRNA into rats to reverse Diabetes-Insip...
bFunctional mRNA half-lifeQ is defined as thetime required to reach 50% of maximum luciferase (LUC)production. Use of this assay might sidestep the question,however, as it fails to take into account the possibility that arapid phase of degradation might occur before mRNAsbecome established on ...
2.4.4. NMD Luciferase Reporter Assay In this study, we used a highly sensitive and quantitative NMD dual luciferase reporter system by measuring WT- and PTC-containing β-globin mRNA levels [7]. pCDNA6-RSV-Renilla-Luc-β-globin (WT) (Renilla-WT) is expressed independent of NMD in the cel...
When GRK1 and rhodopsin are in close proximity, proteolytic release and nuclear localization of tTA occurs, inducing the expression of a luciferase reporter as an indirect readout of the colocalization. The in vitro assay was a fluorescence-based AlphaScreen assay to directly measure either GRK1 ...
Expression of a dominant-negative p97 protein lacking ATPase function resulted in a significant reduction of SP activity in a reporter assay [154,155]. We recently developed a retrotranslocation assay based on expression of biotin ligases and SP fusion to a biotin affinity peptide (BAP) tag. ...
Overexpression of TP53 increased Pol η mRNA levels and enhanced luciferase activity in a reporter assay. Importantly, overexpression of a mutant TP53 (R175H) was unable to enhance luciferase expression. Unpublished data from our laboratory and other studies have confirmed higher expression levels of ...