[求助]ARE luciferase reporter assay，Luciferase值和内参值同时下降。已有1人参与 实验设计，使用 lip...
The expression and transcriptional activity of NRF2 in human HCC biopsies and camptothecin-treated culture cells were determined using immunostaining, western blot, reverse-transcription quantitative real-time PCR (RT–qPCR) and luciferase reporter assay. The effect of camptothecin on chemosensitivity of ca...
AREB6-Luc萤光素酶报告基因质粒(AREB6 luciferase reporter plasmid )是翌圣生物自主研发的用于检测AREB6激活水平为目的的报告基因。 AREB6又称ZEB1是一种锌指结构转录因子,它编码的蛋白在IL-2的转录抑制中起重要作用。另外,AREB6还与肿瘤的发生、发展、凋亡和侵袭转移等生理活动密切相关。
In an antioxidant response element (ARE)-luciferase reporter assay, afzelin increased ARE-luciferase reporter activity in a concentration-dependent manner. Consistently, the expression of antioxidant genes, including NF-E2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and catalase, was enhanced...
Testing the putative 8mer in a luciferase reporter assay revealed a modest but significant repression of relative luminescence (Fig. 3f). Collectively, this suggests that agotrons are a new class of functional Ago-associated non-coding RNAs with miRNA-like capabilities. Agotrons are Dicer ...
Finally, the mechanism of JNK activating Nrf2/ARE signaling pathway was determined by co-immunoprecipitation and ARE-luciferase reporter gene assay. Results The expression levels of JNK1/2 were negatively correlated with aging process. JNK1-β2, JNK2-α1, and JNK2-β2 played important roles in...
b, Forest plot of mutant LXRα activity in a co-expression assay. WT and mutant LXRα and an LXR luciferase reporter construct were transfected into HEK293 cells. At 24 h later luminescence was measured as an index of LXRα activity. Assuming normal distribution, significant increases and ...
S5A). Moreover, to assess viral transcription initiation, dual luciferase reporter constructs containing the 5’ UTRs of early and late viral ORFs were transfected into SFPQ overexpression HEK-293T cell lines and wildtype HEK-293T cells, and luciferase activity was measured. The SFPQ ...
Luciferase Reporter Gene Assay Kit 萤火虫荧光素酶报告基因检测试剂盒 11401ES60/76/80 100 T/500 T/1000 T pGM-CMV Luciferase Reporter Plasmid Positive Control pGM-CMV-Luc荧光素酶报告基因质粒阳性对照 11556ES03 1μg Luciferase Reporter Plasmid negative control (荧光素酶报告基因质粒阴性对照) ...
Through the combination of Western blotting, RNA pull-down, dual-luciferase reporter assay, immunohistochemistry, and immunofluorescence microscopy, this study identified a unique circular RNA arising from the alternative splicing of the keratin 4 gene, which we have named circKrt4. ...