Clinical Application of DNA Sequencing: Sanger and Next-Generation PlatformsCancer is a disease of the genome and all cancers arise due to alterations in DNA that allow the cell to override the checks and balances that are essential for controlled growth and differentiation. To date, the clinical...
The next-generation sequencing (NGS) technology is far more powerful than any existing generic DNA fingerprinting methods in generating DNA markers. In this study, we employed a grain legume crop Lupinus angustifolius (lupin) as a test case, and examined the utility of an NGS-based method of ...
ECORISEQUENCINGDNAA method of directly sequencing for double strandDNA by using Tc resistance gene of pBR322 as templateand oligonucleotide 5'-GGCCCTTTCGTCTTC-3'as primerwhich is located on the adjacent to the EcoRI sitewith the counterclockwise was presented.癌变.畸变.突变...
With the extensive application of PCR technology and DNA sequencing technology,analysis of molecular data has become increasingly important in biological research.That genomic DNA contains rich genetic information renders nuclear markers or combination of nuclear and mitochondrial markers as useful means to ...
21世纪以来,DNA测序技术取得革命性突破,新一代高通量测序技术将成为一种常规手段研究土壤微生物多样性,类似于目前土壤氮磷钾等营养元素的化学分析,成为土壤质量评价的... 郑燕,贾仲君 - 全国稳定同位素新技术开发与应用交流研讨会 被引量: 30发表: 2013年 Next generation sequencing and stable isotope probing of ...
The use of pharmacogenomics in clinical practice is becoming standard of care. However, due to the complex genetic makeup of pharmacogenes, not all genetic variation is currently accounted for. Here, we show the utility of long-read sequencing to resolve
PCR products are radiolabelled with 32P or 33P via end-labelling or PCR incorporation, and separated on a polyacrylamide sequencing gel prior to autoradiographic visualisation. A typical reaction yields 20-100 bands per lane depending on the species and primer. We have used ISSR fingerprinting in...
Volume 2. New High Throughput Technologies for DNA Sequencing and Genomics Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into todays multibillion doll...
One powerful method to define target sequence specificity for this important group of transcription regulators is chromatin immunoprecipitation (ChIP) with microarray chips (chip), commonly called ChIP-chip. A more recent variation of this technique is ChIP-seq where DNA sequencing replaces the ...
Single Cell Sequencing and Systems Immunology Kenian Chen, Xiaomin Dong & Jiaqian Wu Part of the book series: Translational Bioinformatics ((TRBIO,volume 5)) 2805 Accesses 2 Citations Abstract There has been long-standing interest in developing procedures for profiling single-cell gene expression...