A combination of three of the expression proteins whose DNA sequences were isolated by screening a lambda gt11 gene bank with a monospecific antiserum against the protective 41 kD antigen band from P.falciparum completely protect aotus monkeys from P.falciparum infection in model experiments....
So-called "next-generation" sequencing technologies enable rapid generation of data by sequencing massive amounts of DNA in parallel using diverse methodologies which overcome the limitations of Sanger sequencing methods used to sequence the first human genome. Despite opening new frontiers of genomics ...
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2DNA materials possess the potential for nanofabrication due to its characteristic nature, i.e., high precision of Watson–Crick base-pairing and high controllability.1,3,4,5In 1980s, Seeman et al.4proposed the rules for the synthesis of DNA ...
Nucleic acid hybridization with a labeled probe is the only practical way to detect a complementary target sequence in a complex nucleic acid mixture. The first section of this article covers quantitative aspects of nucleic acid hybridization thermodynamics and kinetics. The probes considered are oligonu...
The present invention relates to any DNA sequence comprising as a coding region all or part of the nucleotidic sequence coding for a mRNA coding coding for a cinnamoyl CoA reductase (CCR) in lucern an
Aptamers are obtained by a combinatorial selection method known as systematic evolution of ligands by exponential enrichment (SELEX), in which DNA molecules with the desired binding properties are isolated from a library containing as many as 1015 random sequences. What makes aptamers unique and ...
The present invention relates to an expression system comprising a DNA sequence encoding a polypeptide which has a biological activity of human λ-casein, the system comprising a 5'-flanking sequence capable of mediating expression of said DNA sequence. In preferred embodiments the 5'-flanking sequen...
DNA polymerase choice PCR parameters Primer design:In designing mutagenic primers, it is desirable to place the mutated sequence near the middle of the primer, or at least 7–8 nt away from the 3′ end. This allows efficient 3′ extension...
Aligning and merging fragments of DNA sequences (reads) into contigs without using previous knowledge of the sequence Also known as Ka/Ks or o; the rate of non-synonymous nucleotide substitutions divided by the rate of synonymous nucleotide substitutions. Can be used as an indicator of the ...