NGS technology is the next stage in the evolution of DNA sequencing, allowing for the creation of hundreds to millions of DNA sequences in a short period. The relatively rapid adoption and effectiveness of NGS in research transformed genomes and medical diagnosis. The current method of medical ...
DNA sequencing has led to the discovery of point mutations in DNA sequences that result in mutated, faulty proteins, problematic promoter signals, and genetic variations that have been determined to be the basis of genetic diseases and the basis for human identification. Traditional Sanger sequencing...
We provide an overview of the capabilities and clinical applications of DNAsequencing technologies to raise awareness among researchers about the power of these novel genomic tools. In addition, we discuss that key sequencing principles provide a comparison between existing and near-term technologies and...
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This label-free method for sequencing at the single-molecule level is an achievement on its own. However, nanopores also show promise for the technologically ...
DNA analysis by mass spectrometry: applications in DNA sequencing and DNA diagnostics. Nucleosides Nucleotides 16, 563- 571.H. Koster, D. van den Boom, A. Braun, A. Jacob, C. Jurinke, DPLittle, and K. Tang, "DNA analysis by mass spectrometry: applications in DNA sequencing and DNA ...
Just as Sanger sequencing ushered in a paradigm shift that enabled the molecular basis of biological questions to be directly addressed, to an even greater degree, ultra-high-throughput DNA sequencing is poised to dramatically change the nature of biological research. New sequencing technologies have ...
Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods, together with associated computational tools and the growing availability of public data resources, are transforming drug discovery and development. New opportunities are emerging in target identification owing to improved...
Cloning, sequencing and function ofsanA, a gene involved in nikkomycin biosynthesis ofStreptomyces ansochromogenes A 6 kb DNA fragment which can genetically complement NBB19 was cloned when screening the library for antifungal activity. Sequence analysis showed that the... Junyong,Jia,Wenli,... ...
(2006). The emerging science of epigenom- ics. Hum. Mol. Genet. 15(Spec No 1), ... PA Callinan,AP Feinberg - 《Human Molecular Genetics》 被引量: 513发表: 2006年 New strategies and emerging technologies for massively parallel sequencing: applications in medical research A variety of ...
NGS is comprised of three basic steps: (1) genomic DNA fragment library construction; (2) DNA amplification by polymerase chain reaction (PCR); and (3) sequencing5. Three major NGS platforms: 454 (Roche), Solexa (Illumina) and SOLID (ABI), have allowed wider application of genome ...