In genomic analysis, next-generation sequencing (NGS) technology continues to bring cost-effectiveness, unparalleled sequencing speed, with high promise, and accuracy. NGS technology is the next stage in the evolution of DNA sequencing, allowing for the creation of hundreds to millions of DNA ...
Since the initial publication of the next-generation sequencing (NGS) study in atherosclerosis, the genetic landscape of atherosclerosis has been extensively characterized [12]. By detecting the DNA or RNA information of individual cells in atherosclerosis, NGS reveals cellular heterogeneity in tissues ...
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This label-free method for sequencing at the single-molecule level is an achievement on its own. However, nanopores
Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods, together with associated computational tools and the growing availability of public data resources, are transforming drug discovery and development. New opportunities are emerging in target identification owing to improved...
of DNA, LRS typically analyzes native DNA; therefore, it may be regarded as 5-base sequencing, with the ability to determine the methylation status of CpG sites in addition to the standard four nucleotides identified by SRS. Currently, LRS platforms capable of 5-base sequencing are primarily ...
For instance, sequencing of PCR amplicons is one approach for studying single-nucleotide variations (SNVs) and single-nucleotide polymorphisms (SNPs). High-fidelity DNA polymerases are strongly recommended to prevent introduction of unwanted mutations duri...
Single-cell sequencing in cancer research Genome-wide single-sequencing investigations have the potential to classify individual within a mass. In recent years, various single-DNA and RNA quantific... M Mato Prado,AE Frampton,J Stebbing,... - 《Expert Review of Molecular Diagnostics》 被引量: ...
of three-dimensional conformation of genomes. Self-chromosomal conformation capture (3C) technology has been developed, and 3D genomics and related fields have developed rapidly. In addition, chromatin interaction analysis techniques developed by 3C technologies, such as paired-end tag sequencing (ChIA-...
Since the advent of next-generation sequencing (NGS) in 2005, there has been an explosion of published studies employing the technology to tackle previously intractable questions in many disparate biological fields. This has been coupled with technology development that has occurred at a remarkable pac...
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has advanced our understanding of the pathogenesis of disease and provided valuable insights into new diagnostic and therapeutic strategies. With the expansion of capacity f