JUL 2004, SIRNA and antisense RNA mediated knockdown of mammalian PrP expressiondoi:10.1016/S0197-4580(04)81528-8Hill, Andrew F.Sen, HanWhite, Anthony R.Sharples, RobynSexton, PatrickCollins, Steven J.Cappai, RobertoMasters, Colin L.Elsevier Inc.Neurobiology of Aging...
siRNA就是RNAi,与DNA配对可引导gene editing。 microRNA:早期方法和现有数据相结合或许是一个突破点。需要软件的局限性和偏向性。 piRNA:在低等和节肢动物中出现多。基因组上成簇存在,乒乓模型,调控翻译。 LncRNA: antisense RNAs:反RNA cis-NATs:两个位置在局部 trans-NATs两个位置在远处 如果需要功能互斥,则基因...
Two central molecules,microRNA (miRNA) and small interference RNA (siRNA), are involved in the RNAi pathway. An enzyme called Dicer induces this pathway. Dicer cleaves long double-stranded RNA into short double-stranded fragments (21 nucleotides siRNA). Then each siRNA is unwound into two...
Fragile X-associated tremor/ataxia syndrome (FXTAS) is an incurable neurodegenerative disorder caused by expansion of CGG repeats in the FMR1 5’UTR. The RNA containing expanded CGG repeats (rCGGexp) causes cell damage by interaction with complementary D
Using untransfected cells as reference, the increase in proliferation was higher for siRNA than ASO (20–47% vs. 7–18%), but the data must be carefully interpreted with respect to nonspecific effects on gene expression by siRNA. Our findings of transient p21 gene silencing represent a basis...
RNAse H-mediated cleavage of the RNA strand from the RNA-DNA duplex results in the mRNA being unable to be translated to protein [214]. Antisense oligonucleotides, like siRNA, allow for a targeted approach to MDR1 gene-silencing and should decrease non-specific cytotoxic effects, which are ...
Although the detailed binding mode of DNA/RNA vs. parallel PNA has not been fully revealed, a very well-organized duplex has been depicted via in-house molecular modeling (Fig. 7a), carried out using the density functional tight-binding GFN1-XTB method with the aqueous environment represented ...
Modifications in RNA structure may be tailored as described for antisense nucleic acids. A process for inhibiting expression of the Survivin and/or the HBXIP gene in a cell comprises introduction of an siRNA with partial or fully double-stranded character into the cell. Inhibition is sequence-...
The LNAs antisense oligonucleotides and siRNA sequences are listed in Supplementary Table 1. For overexpression experiments, a biotin-labeled HIFAL lncRNA or an antisense control was directly introduced into cells by lipofectamine transfection. Briefly, a biotin-labeled HIFAL lncRNA or an antisense ...
We have recently proposed a mechanism based on double stranded RNA formation and endo-siRNA processing in a perspective paper that may explain how epigenetic modifications can be targeted either to the sense or the antisense strand [31]. In that respect it is intriguing, that the kidney cell ...