下列种类的RNA中,能起基因表达调控作用的RNA是:A.mRNAB.antisense RNAC.tRNAD.miRNA(microRNA)E.rRNA F. snRNA
Two central molecules,microRNA (miRNA) and small interference RNA (siRNA), are involved in the RNAi pathway. An enzyme called Dicer induces this pathway. Dicer cleaves long double-stranded RNA into short double-stranded fragments (21 nucleotides siRNA). Then each siRNA is unwound into two...
(2011). miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA. EMBO J. 30, 4414- 4422.Hansen TB,Wiklund ED,Bramsen JB,et al. miRNA -dependent gene silencing involving Ago2-mediated cleavage of acircular antisense RNA[J]. EMBO,2011,30(...
主题分类:RNA 关键词:CDR1;环状RNA;miR-671;天然的反义转录;细胞核的microRNA 简介 众所周知,通过结合细胞质中mRNAs靶点的3’端UTR上,microRNA(miRNA)可以调控基因的表达,从而导致mRNA的不稳定性和抑制转录(堪富利士等人,2005年;皮莱等人,2005年;Petersen等人,2006年;Chendrimada等人,20007年)。越来越明显的是...
Although the detailed binding mode of DNA/RNA vs. parallel PNA has not been fully revealed, a very well-organized duplex has been depicted via in-house molecular modeling (Fig. 7a), carried out using the density functional tight-binding GFN1-XTB method with the aqueous environment represented ...
The formed DNA-RNA or RNA-RNA duplexes are then recognized by cellular enzymes (e.g. RNase-H) which degrade the mRNAs, ultimately leading to a decrease in the cellular levels of the protein. So far, the most use of antisense oligonucleotides in vivo has been in the central nervous ...
Issues and cellular total RNA were extracted using Trizol (Catalog No. 16096020, Thermo Fisher Scientific, New York, USA). mRNA was reverse transcribed to cDNA using a reverse transcription kit (Catalog No. RR047A, Takara, Japan). miRNA was synthesized to cDNA using a PolyA tailing kit (Cat...
miRNA: microRNA MMC: Megaspore mother cell mRNA: Messenger RNA M-type MADS: MCM1, AG, DEFA and SRF genes family NAC: NAM, ATAF and CUC family NZZ/SPL: Sporocyteless/nozzle SAP: Sterile apetala sRNA: Small RNA SSDEA: Stage-specific differential expression analysis ...
An antisense lncRNA of HIF-1α (ENST00000554254.1), which we named HIFAL (HIF Antisense LncRNA), was most prominently upregulated in the hypoxic cells and in breast cancer tissues (Fig. 1c and Supplementary Fig. 1d). In addition to HIFAL, two other HIF antisense lncRNAs were ...
Different functions have been defined for antisense lncRNA transcripts including chromatin remodeling of the target gene, regulation of the alternative splicing of the sense transcript, miRNA sponging, and masking of miRNA binding site on sense RNA [27]. The antisense transcripts can either repress ...