4. A method of inducing cell death, or inhibiting growth and/or proliferation of a tumor cell expressing tissue factor, comprising administering, to an individual in need thereof, an effective amount of an antibody drug conjugate comprising an antibody comprising a heavy chain variable region (VH...
Griebenow N, Dilmaç AM, Greven S, Bräse S. Site-specific conjugation of peptides and proteins via rebridging of disulfide bonds using the thiol–yne coupling reaction. Bioconjug Chem. 2016;27:911–7. ArticleCASPubMedGoogle Scholar Koniev O, Dovgan I, Renoux B, et al. Reduction–reb...
“CD70 (TNFSF7) is expressed at high prevalence in renal cell carcinomas and is rapidly internalised on antibody binding” British J. of Cancer. 95:298-306 (2006). Agathanggelou et al., “Expression of immune regulatory molecules in Epstein-Barr virus-associated nasopharyngeal carcinomas ...
Conjugation of MMAE to CTX through this linker was achieved with excellent efficiency, affording a DAR of 3.9, based upon UV-VIS (Fig. 1b), HIC, and SDS-PAGE analysis (Supplementary Fig. 1), and an impressive 83% yield. Fig. 1: Synthesis and characterisation of CTX-MMAE. a ...
Again from the same research group, this was again confirmed in a separate study with respect to the conjugation of trastuzumab to cationic emulsions because the trastuzumab immunoemulsion was significantly bound to the well-known breast cancer cell line SKBR-3 overexpressing the HER2 receptor (...
cell responses, and there was an inadequate response of Th2 or CD8+T-cell (Corbett et al.2020a,b) . Nature published the preclinical trial data. They immunize the mice model by administering intramuscularly with either 0.01 μg, 0.1, or 1 μg dose of vaccine. It was shown in the ...
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Antibody-drug conjugates: Recent advances in conjugation and linker chemistries. Protein Cell 2016, 9, 33–46. [CrossRef] 21. Su, Z.; Xiao, D.; Xie, F.; Liu, L.; Wang, Y.; Fan, S.; Zhou, X.; Li, S. Antibody-drug conjugates: Recent advances in linker chemistry. Acta Pharm....
This study demonstrated how the conjugation site influenced the stability of the linker, where light chain conjugation was cleaved faster than if conjugated to the heavy chain in mice; but the linkers were stable in rats. Moreover, conjugation to the heavy chain resulted in dramatically altered ...
Another T-DM1 resistance model had increased cellular accumulation, relative to sensitive cells, but maintained resistance. In gastric cancer, Wang et al. showed that T-DM1 accumulated significantly less DM1 metabolites in T-DM1-resistant N87KR cell lines relative to the parental N87 cells [82]...