Antibody conjugation is the precise art of attaching a molecule like a fluorescent dye, hapten, oligonucleotide, or enzyme to an antibody and serves as a cornerstone for advanced research. By enabling the direct or indirect detection of proteins, our conjugation process is designed to amplify the ...
A New Direction in Cell Therapies: Antibody-Cell ConjugationRandi Hernandez
Anti-TRBC1 CAR T cells (2.5 × 104) were incubated with 2.5 × 104(1:1) or 5 × 104(1:2) or 12.5 × 104(1:5) Jurkat cells, in the presence or absence of normal T cells. After 48 h, flow cytometry was used to assess GFP and NGFR expression. Numbers be...
Lipid conjugation of Atg8 and its mammalian homologs requires Atg3 (Apg3p/Aut1p in yeast), an ubiquitously expressed E2-like enzyme (5-7). Following C-terminal cleavage by the cysteine protease Atg4, the exposed glycine residue of Atg8 binds to the E1-like enzyme Atg7, is transferred to ...
Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-specific hits. Here, we address this bottleneck by developing a workflow combining cell-free DNA template generation, cell-free protein synthesis, and binding measurements of antibody fragments in a process that ta...
TargetGeneric name (trade name)ConjugationOriginIsotype (conjugate)Cancer(s) treatedInitial approval Cancer cells CD20 Rituximab (Rituxan)115,116 Unconjugated Chimeric IgG1 Chronic lymphocytic leukemia, non-Hodgkin’s lymphoma 1997 Ibritumomab tiuxetan (Zevalin)117 RIT Murine IgG1 (90Y) Non-Hodgkin’s...
The STING agonist (STINGa) and the STINGa scaffold-linker (reagent required for conjugation of the payload to the antibody) were prepared as previously described (compound 11 in US Patent No. 11,155,567, issued Oct 26, 2021, and compound 7 in PCT Patent Application WO2021202984A1, publishe...
Peptide Conjugation Services Peptide Oligo Conjugate Stapled Peptide Stable Isotope Labeled Peptides MAP Peptide Synthesis D Amino Acid Peptide Click Chemistry Rush Peptide Service Peptide Library Gene/Protein Service Antibody Service Chemical Synthesis Catalog ProductsCustom...
We demonstrate that these ADCs are of near complete conjugation and exhibit potent cell killing activity and in vitro plasma stability. Moreover, we generated an ADC conjugated at both light and heavy chains, resulting in a DAR close to 4. With the increased number of payloads, the resultant...
The amide bonds were used as linkers of C8Pt(IV) and Cet via simple chemical conjugation to avoid the complicated linker design in a typical ADC (Scheme 1). In addition, Cet-C8Pt(IV) was found to have a better tumor-killing ability than CisPt. Furthermore, metabolomic analysis has ...