including the characteristics of the antibody itself and buffers or preservatives that may be present along with the antibody sample. Some researchers choose to conjugate their own antibodies, either to save money or because they do not find commercially...
The present disclosure provides methods of using the antibody-drug-conjugate of Formula (I). Specifically, the present disclosure provides methods for reducing target-mediated cross-reactivity using antibody-drug-conjugates (ADCs) of Formula (I). The present disclosure also includes methods of using ...
Antibody Drug Conjugates (ADCs) have a highly specific mechanism of action which is an advantage for the treatment of several oncology indications. They are made of a highly toxic payload, a linker, and an antibody. Usually, they are classified as Highly Potent APIs (HPAPIs) compounds due to...
5. Type of conjugate –The choice of the conjugate for a secondary antibody primarily depends on two factors: (i) the application in which the secondary antibody will be used, and (ii) the detection method that will be used in that application. An example of this is when performing a wes...
5. Type of conjugate– The choice of the conjugate for a secondary antibody primarily depends on two factors: (i) the application in which the secondary antibody will be used, and (ii) the detection method that will be used in that application. An example of this is when performing a wes...
We recommend that you use 5% milk in TBST, shaken for one hour at room temperature, to block all of our non-conjugate primary antibodies. This includes phospho-specific and total antibodies. Now I can already hear the clicking, comments, and hashtags, asking about "what about the phosphatases...
Using a secondary antibody makes it easy to choose a different conjugate no matter the primary antibody. For example, the same primary can be used with a secondary antibody conjugated to HRP in a western blot and with a different secondary antibody conjugated to Alexa Fluor ...
antibody-drug conjugate. This drug targets a certain marker (called CD30) that is more common on some types of lymphoma cells than on healthy cells (Berger 2017). Ibritumomab tiuxetan (Zevalin) is another antibody-drug conjugate. It combines rituximab (Rituxan), an antibody that binds to the...
There are many different ways to detect a secondary antibody, with the choice of conjugate dictated by your detection technology. For western blotting and ELISA applications, enzyme-conjugated secondary antibodies such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) are typically utilized,...
To confirm that the biological sample matrix is not masking or enhancing the signal, spike-and-recovery and linearity-of dilution experiments should be performed. 5) The Detection Antibody Concentration Prepare different concentrations of the detection antibody. 6) The Enzyme Conjugate Concentration ...